Effects of isoprothiolane on cell growth of cultured bovine mammary epithelial cells. |
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| Authors: | H Okada Y Miyake H Ohtsuka Y Kiku S Fukuda A Watanabe Y Yokomizo T J Rosol T Yoshino |
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| Institution: | Department of Veterinary Pathology, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan. |
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| Abstract: | This study was performed to investigate the effects of isoprothiolane on cell growth and the production of interleukin (IL)-1 and IL-6 by bovine mammary epithelial cells in vitro. Isoprothiolane increased proliferation of mammary epithelial cells in a dose-dependent manner at the concentration of 0.05 to 5 microM when cultured either with or without serum-supplemented medium. In contrast, isoprothiolane (0.0005-5 microM) significantly inhibited the production of IL-1 and IL-6 by mammary epithelial cells. Moreover, the cytokines, IL-1alpha, IL-1beta, IL-6, and tumor necrosis factor (TNF)-alpha tended to inhibit the proliferation of mammary epithelial cells in a dose-dependent manner. These results indicated that isoprothiolane regulated mammary epithelial cell growth in vitro possibly by modulating the production of cytokines. |
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