| 黑熊四川亚种线粒体DNA cytb基因的克隆及全序列分析 |
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| 引用本文： | 白素英,唐丽玉,周彩莉.黑熊四川亚种线粒体DNA cytb基因的克隆及全序列分析[J].经济动物学报,2004,8(2):98-101. |
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| 作者姓名： | 白素英 唐丽玉 周彩莉 |
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| 作者单位： | 1. 东北林业大学野生动物资源学院,黑龙江,哈尔滨,150040;国家林业局野生动植物检测,中心,黑龙江,哈尔滨,150040
2. 东北林业大学野生动物资源学院,黑龙江,哈尔滨,150040 |
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| 基金项目： | 东北林业大学科研基金项目 |
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| 摘 要： | 采用PCR克隆技术对黑熊四川亚种线粒体DNA cytb基因进行克隆、测序。黑熊四川亚种线粒体DNA cytb基因的全序列为1140 bp,CG含量为46.23％。与AB020910和U23558两个黑熊cytb序列的变异共有61处.其中T-C转换共有36处.A—G转换共有23处。仅有2处发生T-A颠抉,碱基突变率为5.35％.其中转换率96.72％,颠换率为3.28％。密码子第3个碱基发生变异的有48个。
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| 关 键 词： | 黑熊 线粒体DNA 细胞色素b基因 克隆 序列分析 |
| 文章编号： | 1007-7448(2004)02-0098-04 |
| 修稿时间： | 2004年2月20日 |
Cloning and Sequence Analysis of mtDNA Cytb Gene of the Asian Black Bear |
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| BAI Su-ying,TANG Li-yu,ZHOU Cai-li.Cloning and Sequence Analysis of mtDNA Cytb Gene of the Asian Black Bear[J].Journal of Economic Animal,2004,8(2):98-101. |
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| Authors: | BAI Su-ying TANG Li-yu ZHOU Cai-li |
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| Abstract: | An Asian black bear (Ursus thibetanu mupinensis) mtDNA Cytb gene was amplified, cloned and sequenced. The full length of the black bear mitochondrial DNA Cytb gene (1140bp) is obtained, which C + G content accounts for 46.25 % . The comparison of the nucleotide sequence obtained in this experiment among the sequences downloaded from GenBank( AB020910 and U235S8) showed 61 mutations , including 36 transpositions of T - C, 23 A - G and 2 T - A. The rate of base mutation and transposition of T-C ,A-G&T-Ais5.35% and 96.72 % , 3.28 % respectively. 48 mutation sites were found at the 3rd of base of codons. |
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| Keywords: | black bear mtDNA cytb gene cloning sequence analysis |
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