HPLC法检测乳品中对羟基苯甲酸酯类和纳他霉素

建立了乳制品中的对羟基苯甲酸酯类和纳他霉素的高效液相色谱分析方法.样品用甲醇提取后,使用C18反相色谱柱分离,以甲醇-醋酸水溶液为流动相,紫外检测器检测,检测波长256nm和305nm条件下5种防腐剂在35min之内完全分离.在一定条件下各组分的相关系数均大于0.9990,线形范围1～100mg/kg,回收率为83.7％～103％.

Determination of Parabens and Natamycin in Dairy Products by HPLC

A method for determining parabens and natamycin in dairy products by HPLC is described. Samples wereextracted with methanol and separated on a C18 column with methanol-acetic acid solution as mobile phase. UV detectionwas performed at 256 nm and 305 nm. Complete separation of pimaricin and four parabens, methyl 4-hydroxybenzoate,ethyl 4-hydroxybenzoate, propyl 4-hydroxybenzoate and butyl 4-hydroxybenzoate was achieved within 35 min. Underoptimized conditions, the linear ranges for the food antiseptics was 1–100 mg/kg with a correlation coefficient greater than0.9990, and their average recovery rates from blank real samples spiked at three different levels was between 83.7% and103%. This method was simple, rapid and accurate.