| 冰糖橙优良芽变的AFLP分析 |
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| 引用本文： | 曾柏全,甘霖,熊兴耀,邓秀新,邓子牛.冰糖橙优良芽变的AFLP分析[J].江西农业大学学报,2006,28(2):222-225. |
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| 作者姓名： | 曾柏全 甘霖 熊兴耀 邓秀新 邓子牛 |
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| 作者单位： | 中南林业科技大学,生命科学与技术学院,湖南,长沙,410004;湖南农业大学,园艺园林学院,湖南,长沙,410128;华中农业大学,国家作物改良重点实验室,湖北,武汉,430070 |
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| 基金项目： | 湖南省政府基金(A-15)，中南林学院引进人才科研启动基金(101-0288)共同资助 |
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| 摘 要： | 应用AFLP技术,筛选了40对引物,其中EcoR I AGC M seI CGA,EcoR I AGC M seI CAC,EcoR I AGG M seI CAC,EcoR I AGG M seI CCA,EcoR I ACG M seI CAC,EcoR I ACG M seI CCA等共12对引物经PCR扩增,效果理想,共扩增出4 227条带,带型清晰、丰富、可靠性强。在EcoR1-ACG M seI-CAC这对引物下,果实性状优良的单株变异与普通对照(即普通冰糖橙)有明显的带型差异,多态性比例达10.2%。应用AFLP技术进行柑橘芽变的分析和鉴定获得成功。
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| 关 键 词： | 冰糖橙 AFLP 芽变 |
| 文章编号： | 1000-2286（2006）02-0222-04 |
| 收稿时间： | 2005-12-12 |
| 修稿时间： | 2005年12月12 |
Analysis of Bingtang Orange's Good Bud Mutation by AFLP |
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| ZENG Bai-quan,GAN Lin,XIONG Xing-yao,DENG Xiu-xin,DENG Zi-niu.Analysis of Bingtang Orange's Good Bud Mutation by AFLP[J].Acta Agriculturae Universitis Jiangxiensis,2006,28(2):222-225. |
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| Authors: | ZENG Bai-quan GAN Lin XIONG Xing-yao DENG Xiu-xin DENG Zi-niu |
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| Abstract: | Forty pairs of AFLP primers were adopted in the AFLP analysis.4227 clean and stable bands were detected by twelve pairs of AFLP primers which were selected from 40 pairs of primers.There was obvious band difference between common Bingtang Orange and its bud mutations and excellent fruit under the AFLP primers EcoRI-ACG MseI-CAC,and the polymorphic rate was 10.2%.The application of AFLP in the research of Citrus bud mutation is successful. |
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| Keywords: | Bingtang Orange AFLP bud mutation |
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