愈伤组织辐射诱变选育玉兰新品系

 玉兰茎尖生长点在MS+ 环烷酸钠0. 1 mg/ L+6-BA 1. 5 mg/ L+ 2, 4-D 1 mg/ L+蔗糖45 g/ L 培养基上诱导的愈伤组织, 进行2. 475×10^-10C/ kg г射线辐射处理后, 接种在MS+ ZT 2 mg/ L+ 2, 4-D 1 mg/ L 培养基上, 分化出具有多种变异性状的不定苗。对具有优良性状的不定苗进行生根培养并移栽, 从中选出一株一部分生长旺盛、另一部分与母株基本一样的嵌合体植株。对嵌合体具有优良性状的突变部分进行无性繁殖, 选育出具有一年两次开花、生长速度快而旺盛、抗逆性强等多种优良性状的玉兰新品系。

Selective Breeding of a New Breed of Magnolia denudata from Radiation-induced Mutation of Callus

2. 475×10^-10 C/ kg ray was used to radiate the callus of Magnolia denudata induced from the growing􀀁point in the medium MS+ Sodium naphenate 0. 1 mg/ L+ 6-BA 1. 5 mg/ L+ 2, 4􀀁D 1 mg/ L+ sugar 45 g/L. In the medium MS + ZT 2 mg/ L+ 2, 4-D 1 mg/ L, adventit ious seedlings with variation properties can be differentiated from some treated t issues. Those good seedlings were transplanted into soil and among them a chimaera with one part growing well and the other part basically same as the original parent was selected. A sexual reproduction was carried out on the mutation part with good variat ion characters. As a result, new breed bearing qualities such as growing fast and luxuriantly having strong resistance, blooming twice a year etc. was obtained.