| PYH157广谱抗病基因导入高梁及转基因植株的筛选与研究 |
| |
| 引用本文： | 石太渊,杨立国,王颖,郑文静,高连军. PYH157广谱抗病基因导入高梁及转基因植株的筛选与研究[J]. 杂粮作物, 2001, 21(1): 12-14 |
| |
| 作者姓名： | 石太渊 杨立国 王颖 郑文静 高连军 |
| |
| 作者单位： | 1. 辽宁省农业科学院,生物技术实验室,辽宁,沈阳,110161
2. 辽宁省铁岭市农业科学院,辽宁,铁岭,112616 |
| |
| 摘 要： | 用花粉管通道法将PYH157基因导入高梁，获得了转基因植株。应用卡钠霉素和高粱丝黑穗病鉴定及聚丙烯酰胺等电聚凝胶电泳蛋白质分析表明，无毒广谱抗病基因PYH157已整合到高粱基因组中，并能表达。高粱丝黑穗病田间接种试验中筛选了4个较抗高粱丝黑穗病的转基因植株。
|
| 关 键 词： | PYH157基因 花粉管通道法 高粱 丝黑穗病 抗病育种 转基因育种 |
| 文章编号： | 1003-4803(2001)01-0012-03 |
| 修稿时间： | 2000-07-03 |
Transfering Broad spectrum Resistant PYH157 Gene into Sorghum and Screening Transgenic Plants |
| |
| SHI Tai yuan,YANG Li guo,WANG Ying,ZHENG Wen jing. Transfering Broad spectrum Resistant PYH157 Gene into Sorghum and Screening Transgenic Plants[J]. Rain Fed Crops, 2001, 21(1): 12-14 |
| |
| Authors: | SHI Tai yuan YANG Li guo WANG Ying ZHENG Wen jing |
| |
| Abstract: | By pollen tube pathway, PHY157 gene was transferred into sorghum. Using kanamycin,identification of head smut of sorghum,and polyacrylanide gel electrophoresis confirmed that avirulent PHY157 gene of broad spectrum resistance had been integrated into sorghum and expressed. In comparison with non transgenic plants,four antibacterial silk dust brand transgenic plants had been screened out. |
| |
| Keywords: | PHY157 gene pollen tube pathway sorghum head smut of sorghum |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
