| 农杆菌介导的Vf基因转化柱型苹果的研究 |
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| 引用本文： | 魏海霞,冯涛,王恒振,祝军,戴洪义.农杆菌介导的Vf基因转化柱型苹果的研究[J].安徽农业科学,2007,35(14):4117-4119. |
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| 作者姓名： | 魏海霞 冯涛 王恒振 祝军 戴洪义 |
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| 作者单位： | 青岛农业大学园艺学院,山东青岛,266109;青岛农业大学园艺学院,山东青岛,266109;青岛农业大学园艺学院,山东青岛,266109;青岛农业大学园艺学院,山东青岛,266109;青岛农业大学园艺学院,山东青岛,266109 |
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| 基金项目： | 山东省农业良种工程资助项目. |
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| 摘 要： | 以柱型苹果"鲁加6号"为试材,研究了根癌农杆菌介导的Vf基因转化条件.研究结果表明,该转化的优化条件为:共培养时间为3 d;延迟培养阶段头孢霉素浓度为200 mg/L,时间为6 d;选择培养阶段,芽分化过程卡那霉素浓度为15mg/L,生根过程卡那霉素浓度为10mg/L.PCR检测结果初步表明,外源Vf基因已导入转化植株基因组中.
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| 关 键 词： | 柱型苹果 Vf基因 遗传转化 选择压 |
| 文章编号： | 0517-6611（2007）14-04117-03 |
| 收稿时间： | 2007-02-02 |
| 修稿时间： | 2007-02-02 |
Study on Vf Gene Transformation of Columnar Apple by Agrobacterium tumefaciens |
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| WEI Hai-xia et al.Study on Vf Gene Transformation of Columnar Apple by Agrobacterium tumefaciens[J].Journal of Anhui Agricultural Sciences,2007,35(14):4117-4119. |
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| Authors: | WEI Hai-xia |
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| Institution: | College of Horticuhure, Qingdao Agricuhural University, Qingdao, Shandong 266109 |
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| Abstract: | Transformation systems of columnar apple 'Lujia 6'mediated by A.tumefacience were studied.The result showed that the optimal transformation system was 3 d co-cultivation time,200 mg/L Cef in restoration medium for 6 d,15 mg/L Kan in selection medium during shoot differentiation and 10 mg/L Kan during root formation.PCR analysis result confirmed that Vf gene was integrated successfully into apple genome. |
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| Keywords: | Columnar apple Vf gene Genetic transformation Selection pressure |
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