儿茶素对草鱼生长性能、血清抗氧化指标和肌肉品质的影响

本试验旨在研究儿茶素对草鱼生长性能、血清抗氧化指标和肌肉品质的影响。试验选取平均体质量为(18.5±0.2)g的草鱼360尾,随机分为6组,分别饲喂在基础饲料中添加0(对照组)、0.1、0.3、0.5、0.7和0.9 g/kg儿茶素的6种饲料,每组3个重复,每个重复20尾。试验期60 d。结果表明:1)随着儿茶素的添加,各组间鱼体增重率和饲料系数无显著差异(P>0.05),添加0.5、0.7和0.9 g/kg儿茶素显著提高了鱼体肥满度(P<0.05),显著降低了血清丙二醛含量(P<0.05)。除0.1 g/kg组外,其余各儿茶素添加组血清超氧化物歧化酶活性均显著高于对照组(P<0.05),各组间在血清碱性磷酸酶和过氧化氢脱氢酶活性上无显著差异(P>0.05)。2)在肌肉游离氨基酸和脂肪酸组成方面,0.5、0.7 g/kg组的总氨基酸、呈味氨基酸含量和C20∶5、n⁃3不饱和脂肪酸含量显著高于对照组(P<0.05)。3)各组间在肌肉水分、粗蛋白质、粗脂肪、粗灰分含量及肌肉质构特性、肌肉失水率以及肌纤维密度和直径上差异均不显著(P>0.05),但0.7和0.9 g/kg组的肌肉胶原蛋白含量显著高于对照组(P<0.05)。上述结果显示,儿茶素对草鱼生长性能没有显著影响,可提高血清抗氧化能力,在一定程度上改善肌肉品质。草鱼饲料中儿茶素的推荐添加量为0.5~0.7 g/kg。

Effects of Catechin on Growth Performance,Serum Anti-Oxidation Indicators and Muscle Quality of Grass Carp(Ctenopharyngodon idella)

To investigate the effects of catechin on the growth performance,anti⁃oxidation indicators and mus⁃cle quality of grass carp(Ctenopharyngodon idella),a total of 360 grass carp with an initial body weight of(18.5±0.2)g were selected and randomly divided into six groups,fed six diets with 0(control diet),0.1,0.3,0.5,0.7,0.9 g/kg catechin,respectively,with three replicates per group and 20 fish per replicate.The experimental period was 60 d.The results showed as follows:1)the weight gain and feed conversion ratio showed no significant difference among groups(P>0.05),while the condition factor was significantly in⁃creased and serum malondialdehyde(MDA)content was significantly decreased by the addition of 0.5 and 0.7 g/kg catechin(P<0.05).Dietary catechin significantly increased serum superoxide gasification enzyme(SOD)activity(P<0.05)except the addition of 0.1 g/kg catechin,while alkaline phosphatase(AKP)and hydrogen peroxide dehydrogenase(CAT)activities showed no significant difference among groups(P>0.05).2)The inclusion of 0.5 and 0.7 g/kg catechin significantly increased the total free amino acids and the deli⁃cious free amino acids contents in muscle,and the C20∶5 and polyunsaturated fatty acids(n⁃3PUFA)contents were also significantly increased compared with the control group(P<0.05).3)No significant differences were observed in muscle proximate composition,texture characteristics,water⁃holding capacity and muscle his⁃tology among all groups(P>0.05),but muscle collagen content was significantly increased by the addition of 0.7 and 0.9 g/kg catechin(P<0.05).In conclusion,dietary catechin has no significant effect on growth per⁃formance,but can improve the serum anti⁃oxidant ability and muscle quality of grass carp,and the recommen⁃ded addition of catechin are 0.5 to 0.7 g/kg.