支气管败血波氏杆菌的鉴定及药敏试验

从猪萎缩性鼻炎(AR)临床症状猪群分离出34株疑似支气管败血波氏杆菌(Bb),对分离菌进行形态结构、染色特性、培养性状和生化特性等方面的测定,结果这些分离菌均符合Bb的生物学特征;对BbFlaA基因进行PCR扩增,所有分离菌均能扩增出特异性DNA条带,与传统方法的鉴定结果完全一致,且最小检出量为0.64pg;而猪鼻腔和肺组织中常见的多杀性巴氏杆菌、金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、变形杆菌及枯草芽孢杆菌均未出现任何DNA条带。药敏试验显示,这些分离物对多黏霉素B、丙氟哌酸、氟哌酸、卡那霉素和庆大霉素均表现高度敏感。

Identification and Drug Sensitivity of Bordetella bronchiseptica Solates from Cases of Swine Atrophic Rhinitis

34 isolates were isolated from 156 nasal swabs,which were collected from pig herds showing clinical symptoms of atrophic rhinitis in Guizhou province and those isolates were identified as Bordetella bronchiseptica(Bb)by their morphology and structure,staining reaction,cultural performance and biochemical reaction.The specific DNA fragment was amplified from 34 isolates based on one pair of primer of Bb flagellin gene.The minimum detection content was 0.64 pg.And the primers had no cross reaction with P.multocida,S.aureus,B.subtilis,P.aeruginosa,P.mirabillis and E.coli,which were commonly found in nasal cavity and lung tissue of pig.These isolates were high-sensitive to polymyxin broth,ciprofloxacin,norfloxacin,kanamycin and gentamicin.