Quantitative disease resistance assessment by real-time PCR using the Stagonospora nodorum-wheat pathosystem as a model |
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| Authors: | R P Oliver K Rybak M Shankar R Loughman N Harry P S Solomon |
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| Institution: | Australian Centre for Necrotrophic Fungal Pathogens, Murdoch University, WA 6150;and;Department of Agriculture and Food, 3 Baron-Hay Court, South Perth, WA 6151, Australia |
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| Abstract: | Measuring disease resistance accurately and reproducibly is a key requirement for the introgression of partial resistance genes into breeding lines. Here, a qPCR protocol is used to measure fungal biomass, using the wheat- Stagonospora nodorum pathosystem as a model. Seven cultivars of differing reported resistance levels were used. Fungal biomass taken at 220°C thermal days after inoculation accurately predicts the final grain weight loss. It is concluded that a test based on qPCR methods is specific, quantitative, rapid and objective. Such tests could provide useful and economic tools in the development of robustly resistant crop cultivars. |
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| Keywords: | breeding for disease resistance glume blotch of wheat stagonospora leaf blotch Triticum aestivum |
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