Transposon mutagenesis used to study the role of complement resistance in the virulence of an avian Escherichia coli isolate. |
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| Authors: | L K Nolan R E Wooley R K Cooper |
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| Institution: | Department of Medical Microbiology, College of Veterinary Medicine, University of Georgia, Athens 30602. |
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| Abstract: | The role of complement resistance in the virulence of an avian Escherichia coli isolate was examined with transposon mutagenesis. A suicide plasmid containing a kanamycin-encoding mini-transposon was used to transform a virulent complement-resistant avian E. coli isolate. A less resistant mutant was identified that contained a transposon insertion in a plasmid and in the chromosome. This loss of complement resistance was associated with a drop in virulence in an embryo assay. No other phenotypic changes were detected in the mutant. These results suggest that complement resistance is associated with the virulence of this organism. |
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