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| 狂犬病病毒免疫组织化学定位方法的建立 | |
| 引用本文： | 王泽,夏志平,江禹,连海,毛文俊,钱爱东.狂犬病病毒免疫组织化学定位方法的建立[J].吉林农业大学学报,2009,31(6). |
| 作者姓名： | 王泽 夏志平 江禹 连海 毛文俊 钱爱东 |
| 作者单位： | 1. 军事医学科学院军事兽医研究所,长春,130062;吉林农业大学动物科技学院,长春,130118 2. 军事医学科学院军事兽医研究所,长春,130062 3. 成都军区空军食品基地,成都,610000 4. 吉林农业大学动物科技学院,长春,130118 |
| 基金项目： | 国家自然科学基金项目(30600445) |
| 摘 要： | 应用抗狂犬病病毒的特异性抗体及免疫组织化学方法检测接种了狂犬病病毒SRV-9毒株感染的小鼠脑组织,并对狂犬病病毒抗原进行了组织定位分析。结果表明:在小鼠的小脑分子层蒲肯野氏细胞胞浆出现明显的局灶型阳性颗粒。说明免疫组织化学方法检测狂犬病病毒敏感度高、直观,可作为诊断狂犬病病毒的辅助性方法,对研究狂犬病病毒在脑组织神经细胞及其他组织器官内的分布具有一定的意义。 |
| 关 键 词： | 免疫组织化学 狂犬病病毒 检测方法 |
Establishment of Immunohistochemical Localization of Rabies Virus |
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| WANG Ze,XIA Zhi-ping,JIANG Yu,LIAN Hai,MAO Wen-jun,QIAN Ai-dong.Establishment of Immunohistochemical Localization of Rabies Virus[J].Journal of Jilin Agricultural University,2009,31(6). | |
| Authors: | WANG Ze XIA Zhi-ping JIANG Yu LIAN Hai MAO Wen-jun QIAN Ai-dong |
| Institution: | WANG Ze1,2,XIA Zhi-ping1,JIANG Yu1,LIAN Hai1,MAO Wen-jun3,QIAN Ai-dong21.Institute of Military Veterinary,Academy of Military Medical Sciences,Changchun 130062,China,2.College of Animal Science , Technology,Jilin Agricultural University,Changchun 130118,3.Food Basis for Chengdu Airforce,Chengdu 610000 |
| Abstract: | Rabies virus antigen in suckling brain tissues of mice infected with rabies virus by the specific antibody and immunohistochemistry technique was detected and the localization of rabies virus antigen in the brain tissues was analyed.The results showed that there were positive grains in the molecular layer of cerebellum.We can see that immunohistochemistry technique with labeled specific antibody was sensitive in detecting rabies virus and it can be an adjuvanticity method for detecting rabies virus and has ... |
| Keywords: | immunohistochemistry rabies virus detection method |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |