| 梨种质资源遗传多样性研究中的RAPD技术引物筛选 |
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| 引用本文： | 马艳芝,张玉星. 梨种质资源遗传多样性研究中的RAPD技术引物筛选[J]. 中国农学通报, 2009, 25(11): 30-33 |
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| 作者姓名： | 马艳芝 张玉星 |
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| 作者单位： | 1. 河北农业大学园艺学院,河北保定,071001;唐山师范学院生命科学系,河北唐山,063000
2. 河北农业大学园艺学院,河北保定,071001 |
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| 摘 要： | 以梨属44个种和品种为试验材料，利用RAPD技术从50个随机引物中筛选出18个多态性引物，用于梨种质资源遗传多样性研究。结果表明：所选出的多态性引物每个引物扩增出的条带数在4—11之间，扩增出的DNA片段分子量大多在450bp—2000bp之间，共扩增出118个条带，其中样品间相同的条带数有11条，呈现多态性的条带107条，多态百分率达90.8%。
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| 关 键 词： | 聚丙烯酰胺 聚丙烯酰胺 沙化草场 颗粒组成 |
| 收稿时间： | 2009-03-06 |
| 修稿时间： | 2009-03-11 |
Screening of Primers in Genetic Diversity Analysis of Pyrus Germplasm by RAPD |
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| MA Yan-Zhi. Screening of Primers in Genetic Diversity Analysis of Pyrus Germplasm by RAPD[J]. Chinese Agricultural Science Bulletin, 2009, 25(11): 30-33 |
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| Authors: | MA Yan-Zhi |
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| Affiliation: | Ma Yanzhi, Zhang Yuxing (1College of Horticulture, Agricultural University of Hebei, Baoding Hebei 071001; 2Department of Biological Science, Tangshan Teacher＇s College, Tangshan Hebei 063000) |
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| Abstract: | 18 primers selected from 50 ones were identified to be high polymorphic and were applied on RAPD amplification of 44 Pyrus plants, for genetic diversity analysis of Pyrus germplasm. The amounts of bands were 4-11 and the molecular weight mainly was 450bp-2000bp. A total of 118 DNA bands were amplified, including 11 same bands and 107 polymorphic bands. |
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| Keywords: | RAPD |
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