| 大豆体细胞胚对卡那霉素和PPT敏感性的研究 |
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| 引用本文： | 刘尚前,王罡,季静,王萍. 大豆体细胞胚对卡那霉素和PPT敏感性的研究[J]. 河北北方学院学报(自然科学版), 2007, 23(1): 9-12 |
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| 作者姓名： | 刘尚前 王罡 季静 王萍 |
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| 作者单位： | 河北北方学院农业科学系,河北,张家口,075000;天津大学农业与生物工程学院,天津,300072;淮海工学院海洋学院,江苏,连云港,222005 |
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| 基金项目： | 国家植物转基因技术研究开发与中试基地建设专项课题(J99-B-001) |
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| 摘 要： | 以大豆(Glycine max(L.)Merrill)品种合丰25、东农L13、东农40、黑农40、黑农35、CH21141、Progress的体细胞胚为材料,采用MS培养基附加2,4-D(15 mg/L~20 mg/L),以不同浓度的卡那霉素和PPT处理.结果表明:大豆对卡那霉素和PPT敏感性在基因型间存在显著差异;随着卡那霉素和PPT浓度的增加,大豆体细胞胚褐化率增加,体积增殖减小;以卡那霉素作为筛选剂,最初使用浓度为50~100 mg/L,而以PPT作为筛选剂,最初使用浓度为2 mg/L.
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| 关 键 词： | 大豆 体细胞胚 抗生素 |
| 文章编号： | 1673-1492（2007）01-0009-04 |
| 修稿时间： | 2006-10-24 |
The Study on the Sensitivity of Somatic Embryo Tissue Treated with Kanmycin and PPT |
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| LIU Shang-qian,WANG Gang,JI Jing,WANG Ping. The Study on the Sensitivity of Somatic Embryo Tissue Treated with Kanmycin and PPT[J]. JournalofHebeiNorthUniversity(NaturalScienceEdition), 2007, 23(1): 9-12 |
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| Authors: | LIU Shang-qian WANG Gang JI Jing WANG Ping |
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| Affiliation: | 1. Department of Agricuhurl Sciences, Hebei North University, Zhangjiakou 075000, Heibei, China; 2. College of Agricultural and Biological Engineering, Tianjin University, Tianjin 300072, China; 3. Marine School, Huaihai Institute of Technology, Lianyungang 222005, Jiangsu, China |
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| Abstract: | The sensitivity of somatic embryo tissue treated with kanmycinand PPT was studied.The results showed that there was certain difference between the sensitivity of soybeancultivars to selective agents;with the concentrations of kanmycin and PPT increased,the brown frequency of somatic embryo tissue increased,but the proliferation of somatic embryo tissue decreased;the suitable concentrations were kanmycin 100mg/L,PPT 2 mg/L. |
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| Keywords: | soybean somatic embryos antibotics |
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