| 黄花忍冬茎和叶中绿原酸的提取及含量测定 |
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| 引用本文： | 胡彦武,于俊林,孙仁爽.黄花忍冬茎和叶中绿原酸的提取及含量测定[J].湖北农业科学,2009,48(11). |
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| 作者姓名： | 胡彦武 于俊林 孙仁爽 |
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| 作者单位： | 通化师范学院制药与食品科学系,吉林,通化,134002 |
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| 基金项目： | 通化师范学院自然科学科研摹金项目 |
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| 摘 要： | 采用超声提取法提取黄花忍冬茎和叶中绿原酸并用HPLC法测定其含量.Phenomsil C18色谱柱(150mm×4.6mm,5μm),流动相为乙腈-0.4%磷酸溶液(13:87),流速为0.8 mL·min~(-1),检测波长为327nm.结果表明,在0.20-1.00μg范围内绿原酸呈现良好的线性关系(r=0.999 8),平均回收率为95.50%,RSD=2.86%.此方法快速简便,重现性好,绿原酸的含量因部位的不同而有差异.
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| 关 键 词： | 黄花忍冬茎和叶 高效液相色谱 绿原酸 舍量测定 |
Extraction and Determination of Chlorogenic Acid in the stems and leaves of Lonicera chrysantha Turcz. |
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| HU Yan-wu,YU Jun-lin,SUN Ren-shuang.Extraction and Determination of Chlorogenic Acid in the stems and leaves of Lonicera chrysantha Turcz.[J].Hubei Agricultural Sciences,2009,48(11). |
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| Authors: | HU Yan-wu YU Jun-lin SUN Ren-shuang |
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| Abstract: | The chlorogenic acid in the steins and leaves of Lonicera chrysantha Turcz. were extracted with ultrasonic extraction and analyzed with HPLC. The separation was preformed on Phenomsil C18 (150 mm×4.6 mm, μm) with acetonitrile0.4%H_3PO_4 (13:87) as mobile phase. The results showed the contents of chlorogenic acid were different in different parts of Lonicera chrysantha Turcz. The linear range was 0.20-1.00 μg (r=0.999 8). The average recovery of chlorogenic acid was 95.50%,RSD=2.86%. As a result, this method was simple, rapid with a good reproducibility. |
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| Keywords: | stems and leaves of Lonicera chrysantha Turcz HPLC chlorogenic acid content determination |
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