| 金边连翘工厂化组培育苗技术研究 |
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| 引用本文： | 王晶,赵爽,唐存莲,左利娟,陈兰芬. 金边连翘工厂化组培育苗技术研究[J]. 河北林果研究, 2010, 25(3): 289-291 |
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| 作者姓名： | 王晶 赵爽 唐存莲 左利娟 陈兰芬 |
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| 作者单位： | 北京农业职业学院,北京102442 |
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| 摘 要： | 以金边连翘的幼嫩茎段为外植体,研究了其工厂化组培育苗技术。结果表明:初代培养最佳培养基配方为MS+6-BA 0.2 mg/L+NAA 0.01 mg/L;MS+6-BA 2 mg/L+NAA 0.2 mg/L作为增殖培养基效果最好。生根阶段的最佳培养基为1/2MS+NAA 0.1mg/L+IBA 0.1 mg/L生根效果最好,用蛭石和珍珠岩1∶1的比例移栽苗时成活率可达95%以上。
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| 关 键 词： | 金边连翘 组织培养 工厂化育苗 |
Industrialized seedling technique of tissue culture of Forsythia Suspensa Jinbian |
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| WANG Jing,ZHAO Shuang,TANG Cun-lian,ZUO Li-juan,CHEN Lan-fen. Industrialized seedling technique of tissue culture of Forsythia Suspensa Jinbian[J]. Hebei Journal of Forestry and Orchard Research, 2010, 25(3): 289-291 |
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| Authors: | WANG Jing ZHAO Shuang TANG Cun-lian ZUO Li-juan CHEN Lan-fen |
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| Affiliation: | (Beijing Vocational College of Agriculture,Beijing 102442,China) |
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| Abstract: | The rapid seedling technique via tissue culture in Forsythia suspensa Jinbian was studied by using new stem as explant.The results showed that the optimal inducing medium for an adventitious bud was MS＋6-BA 0.2 mg/L＋NAA0.01 mg/L;the best medium for successive transfer culture was MS＋6-BA 2 mg/L＋NAA 0.2 mg/L;and the medium of 1/2MS＋NAA 0.1 mg/L＋IBA 0.1 mg/Lwas the better one for asventitious root inducing.Plantlets transplanted on medium which contained vermiculite and perlite with 1∶1 had survival rate of more than 95%. |
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| Keywords: | Forsythia suspensa Jinbian tissue culture industrialized seedling technique |
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