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| 农杆菌介导的洋桔梗遗传转化体系的建立 | |
| 引用本文： | 郑阳霞,黄彬城,季静,王罡,杨婉身.农杆菌介导的洋桔梗遗传转化体系的建立[J].核农学报,2009,23(4). |
| 作者姓名： | 郑阳霞 黄彬城 季静 王罡 杨婉身 |
| 作者单位： | 1. 四川农业大学园艺学院,四川,雅安,625014;天津大学农业与生物工程学院,天津,300072 2. 天津大学农业与生物工程学院,天津,300072 3. 四川农业大学生命科学与理学院,四川,雅安,625014 |
| 基金项目： | 科技部农业科技成果转化资金项目(2006GB2A100013) |
| 摘 要： | 以洋桔梗叶片为外植体,建立了植株再生体系,并在此基础上,用根癌农杆菌介导法,研究了影响洋桔梗转化的若干因素。结果表明,诱导最佳培养基为MS+6-BA 1.0mg/L+NAA 0.15mg/L,不定芽诱导率达74.5%;不定芽生根培养基为1/2MS+IBA 0.5 mg/L,生根率达90%。不定芽分化阶段和生根阶段的Km筛选浓度分别为45mg/L和15mg/L;根癌农杆菌菌液浓度OD600值为0.6,侵染时间10min,共培养时间3d,有利于抗性芽的产生。Km筛选得到的抗性植株经过PCR和Southern检测表明,八氢番茄红素合成酶基因(PSY)已经整合到洋桔梗基因组中。 |
| 关 键 词： | 洋桔梗 植株再生 农杆菌 遗传转化 |
ESTABLISHMENT OF Agrobacterium-MEDIATED TRANSFORMATION SYSTEM OF Eustoma grandiflorum |
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| ZHENG Yang-xia,HUANG Bin-cheng,JI Jing,WANG Gang,YANG Wan-shen.ESTABLISHMENT OF Agrobacterium-MEDIATED TRANSFORMATION SYSTEM OF Eustoma grandiflorum[J].Acta Agriculturae Nucleatae Sinica,2009,23(4). | |
| Authors: | ZHENG Yang-xia HUANG Bin-cheng JI Jing WANG Gang YANG Wan-shen |
| Institution: | 1;2;1.College of Horticulture;Sichuan Agricultural University;Ya'an;Sichuan 625014;2.College of Agriculture and Biotechnology;Tianjin University;Tianjin 300072;3.College of Biology and Science;Sichuan 625014 |
| Abstract: | The regeneration system of Eustoma grandiflorum in vitro was studied with leaf as explant.The results showed that the optimal culture medium for adventitious bud induction was MS+6-BA 1.0mg/L+NAA 0.15mg/L with induction frequency of 74.5%.As for the rooting induction,the best medium with the rooting rate of 90% was 1/2MS+ IBA 0.5 mg/L.The genetic transformation system of Eustoma grandiflorum was established and optimized,which indicated that the optimum concentration of Km for resistant shoots differentiati... |
| Keywords: | Eustoma grandiflorum plant regeneration agrobacterium genetic transformation |
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