| 稀土元素铈对提高细胞培养喜树碱产量的研究 |
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| 引用本文： | 潘学武,董妍玲,石亚亚,许恒皓.稀土元素铈对提高细胞培养喜树碱产量的研究[J].北方园艺,2010(17):91-93. |
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| 作者姓名： | 潘学武 董妍玲 石亚亚 许恒皓 |
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| 作者单位： | 武汉生物工程学院,生物工程系,湖北,武汉,430415;武汉大学,生命科学学院,植物发育教育部重点实验室,湖北,武汉,430072;武汉生物工程学院,生物技术系,湖北,武汉,430415;武汉大学,生命科学学院,植物发育教育部重点实验室,湖北,武汉,430072 |
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| 基金项目： | 湖北省自然科学基金，武汉大学博士后研究资助项目 |
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| 摘 要： | 在喜树悬浮培养细胞中,三价稀土铈(Ce3+)的诱导刺激能显著促进喜树碱的生物合成。在细胞培养的第10天加入0.1mM的Ce3+,可使喜树碱产量达到最大值13.02mg/L,是没有受到刺激的6.39倍。与此相应的是,Ce3+刺激能显著提高培养细胞苯丙氨酸裂解酶的活性,增加细胞内丙二醛的含量。
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| 关 键 词： | 稀土元素 喜树碱 铈 植物细胞培养 |
Improvement of Camptothecin Production with Cell cultures of Camptotheca acuminata Decaisne by Rare Earth Element Cerium |
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| PAN Xue-wu,DONG Yan-ling,SHI Ya-ya,XU Heng-hao.Improvement of Camptothecin Production with Cell cultures of Camptotheca acuminata Decaisne by Rare Earth Element Cerium[J].Northern Horticulture,2010(17):91-93. |
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| Authors: | PAN Xue-wu DONG Yan-ling SHI Ya-ya XU Heng-hao |
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| Institution: | PAN Xue-wu1,3,DONG Yan-ling2,SHI Ya-ya3,XU Heng-hao3 (1.Department of Bioengineering,Wuhan Bioengineering Institute,Wuhan,Hubei 430415,2.Department of Biotechnology,3.Key Lab of MOE for Plant Developmental Biology,College of Life Sciences,Wuhan University,Hubei 430072) |
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| Abstract: | In suspension cultures of Camptotheca acuminata Decaisne,one kind of rare earth elements,cerium(as Ce3+) could significantly elicit and improve the biosynthesis of camptothecin.When the concentration of cerium at 0.1mM was supplemented on the 10th day,maximum camptothecin production was achieved,reaching 13.02mg/L,which was 6.39folds of that of the control.Accordingly,higher phenylalanine ammonia-lyase(PAL)activity and malondialdehyde (MDA)contents of the treated cultures increased as well. |
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| Keywords: | rare earth element Camptotheca acuminata Decaisne cerium plant cell culture |
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