| 应用15N核磁共振技术对苯聚合物结合蛋白中氮形态的研究 |
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| 作者姓名： | CHENG Li-Li WEN Qi-Xiao |
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| 作者单位： | [1]InstituteofSoilScience,AcademiaSinica,P.O.Box821,Nanjing210008,China [2]InstituteofSoilScience,AcademiaS |
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| 基金项目： | *1Project supported by the National Natural Science Foundation of China. |
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| 摘 要： | Phenolic polymers synthesized by reactions by reactions of p-benzoquinone with 15N-labelled protein or (15NH4)2SO4 were studied by using 15N CP-MAS NMR technique in combination with chemical approaches.Results showed that more than 80% of nitrogen in quinone-protein polymers was in the form of amide with some present as aromatic and /or aliphatic amine and less than 10% of nitrogen occurred as heterocyclic N.The nitrogen distribution in the non-hydrolyzable residue of the quinone-protein polymers was basically similar to that of soil humic acid reported in literature with the exception that a higher proportion of N as heterocyclic N and aromatic amine and a lower proportion of N as amide and aliphatic amine were found in the former than in the latter,More than 70% of total nitrogen in quinone-(NH4)2OS4 polymer was acid resistant ,of which about 53% occurred as pyrrole,nitrile and imion type N.The possible roles of the reactions of phenols or quinones with proteins in the formation of humic acid.especially the non-hydrolyzable nitrogen in humicacid,are discussed.
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| 关 键 词： | 腐植酸 氮形态 含蛋白酚醛聚合物 杂环 15NNMR |
Study on nitrogen forms in phenolic polymers incorporating protein by 15N CP-MAS NMR |
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| CHENG Li-Li,WEN Qi-Xiao.Study on nitrogen forms in phenolic polymers incorporating protein by 15N CP-MAS NMR[J].Pedosphere,1996,6(2):97-106. |
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| Authors: | CHENG Li-Li and WEN Qi-Xiao |
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| Institution: | [1]InstituteofSoilScience,AcademiaSinica,P.O.Box821,Nanjing210008,China [2]InstituteofSoilScience,AcademiaS |
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| Abstract: | Phenolic polymers synthesized by reactions by reactions of p-benzoquinone with 15N-labelled protein or (15NH4)2SO4 were studied by using 15N CP-MAS NMR technique in combination with chemical approaches.Results showed that more than 80% of nitrogen in quinone-protein polymers was in the form of amide with some present as aromatic and /or aliphatic amine and less than 10% of nitrogen occurred as heterocyclic N.The nitrogen distribution in the non-hydrolyzable residue of the quinone-protein polymers was basically similar to that of soil humic acid reported in literature with the exception that a higher proportion of N as heterocyclic N and aromatic amine and a lower proportion of N as amide and aliphatic amine were found in the former than in the latter,More than 70% of total nitrogen in quinone-(NH4)2OS4 polymer was acid resistant ,of which about 53% occurred as pyrrole,nitrile and imion type N.The possible roles of the reactions of phenols or quinones with proteins in the formation of humic acid.especially the non-hydrolyzable nitrogen in humicacid,are discussed. |
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| Keywords: | humic acid 15N NMR phenolic polymer |
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