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Fertilisation assessment and postzygotic development in several intra- and interspecific Prunus hybrids
Authors:M J Rubio-Cabetas  R Socias i Company
Institution:(1) Unidad de Fruticultura. S.I.A.-D.G.A., Campus de Aula Dei. Apartado 727, 50080 Zaragoza, Spain
Abstract:Summary the clearing technique was applied to assess fertilisation and to follow the postzygotic development in ten intra- and interspecific crosses among Prunus species used as rootstocks. The technique allowed to distinguish fertilised ovules from unfertilised ones, as well as to calculate the fertilisation percentage in intra-and interspecific compatible crosses. All the stages from unfertilised to fertilised ovules were analysed in order to determine the best time for embryo rescue. Differences in postzygotic development among the crosses are discussed in terms of the genotype of the seed parent in the pollen-pistil interactions.Abbreviations DAP Days After Pollination
Keywords:clearing technique  interspecific hybridisation  Prunus L    postzygotic development  rootstocks  woody plants
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