Comparison of the viral proteins of canine parvovirus-2, mink enteritis virus and feline panleukopenia virus |
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Authors: | PS Carman RC Povey |
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Institution: | 1. Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2W1, Canada;2. Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2W1, Canada |
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Abstract: | Canine parvovirus-2 (CPV-2), Mink enteritis virus (MEV) and feline panleukopenia virus (FPV) were produced using identical cell culture and purification techniques. The distributions of the haemagglutinating activity of the three different parvoviruses in a CsCl gradient were similar with haemagglutinating peaks identified at 1.48–1.49, 1.42, 1.36 and 1.30–1.31 g cm?3. The number and distribution of the viral proteins and the equivalent protein molecular weights are similar for all three viruses in SDS-polyacrylamide gels (10%). Four viral proteins were identified and their molecular weights were determined: protein A (77 500–79 500), protein B (63 000–63 500), protein C (61 500–63 000) and protein D (50 000–55 000). The viral protein D although reported for some other parvoviruses has not previously been demonstrated in CPV-2, MEV or FPV. |
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