| 生防细菌BRF-1和BRF-2鉴定及生物学特征 |
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| 引用本文： | 王光华,金剑,徐美娜,陈雪丽,刘晓冰.生防细菌BRF-1和BRF-2鉴定及生物学特征[J].中国生物防治,2007,23(1):49-54. |
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| 作者姓名： | 王光华 金剑 徐美娜 陈雪丽 刘晓冰 |
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| 作者单位： | 1. 中国科学院东北地理与农业生态研究所/黑土生态实验室,哈尔滨,150081
2. 中国科学院东北地理与农业生态研究所/黑土生态实验室,哈尔滨,150081;黑龙江八一农垦大学植物科技学院 |
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| 基金项目： | 黑龙江省哈尔滨市科技攻关项目;中国科学院前沿领域研究基金 |
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| 摘 要： | BRF-1和BRF-2是从大豆根际土壤中筛选得到的两株革兰氏阳性生防细菌,平板对峙培养表明它们对7种植物病原真菌具有拮抗作用。BRF-1芽孢球型中生,无鞭毛;而BRF-2芽孢球型端生,周生鞭毛。部分生物学特征研究表明,两菌株之间存在较大的差异。采用Biolog微生物自动鉴定仪鉴定和对两菌株的16S rDNA基因序列分析表明,菌株BRF-1和BRF-2分别与多粘类芽孢杆菌和枯草芽孢杆菌亲缘关系最近,故把菌株BRF-1和BRF-2分别定为多粘类芽孢杆菌和枯草芽孢杆菌。
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| 关 键 词： | 多粘类芽孢杆菌 枯草芽孢杆菌 Biolog鉴定 16S Rdna测序 生物学特征 |
| 文章编号： | 1005-9261(2007)01-0049-06 |
| 修稿时间： | 2006-02-27 |
Identification and Biological Characteristics of Biocontrol Bacterial Strains BRF-1 and BRF-2 |
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| WANG Guang-hua,JIN Jian,XU Mei-na,CHEN Xue-li,LIU Xiao-bing.Identification and Biological Characteristics of Biocontrol Bacterial Strains BRF-1 and BRF-2[J].Chinese Journal of Biological Control,2007,23(1):49-54. |
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| Authors: | WANG Guang-hua JIN Jian XU Mei-na CHEN Xue-li LIU Xiao-bing |
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| Institution: | Lab of Black Soil Ecology, Northeast Institute of Geography and Agricultural Ecology, Academia Sinica Harbin 150040, China |
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| Abstract: | BRF-1 and BRF-2 were gram positive biocontrol bacterial strains isolated from soybean rhizosphere.Dual-culture tests showed that the two strains were strongly against seven plant fungal pathogens.Both strains formed endospores.The endospore of BRF-1 and was BRF-2 grown in the middle and at the end of cell, respectively.BRF-1 has no flagellum,and BRF-2 has flagella growing around cell.Research results showed that the biological characteristics of BRF-1 and BRF2 were different.Identification by using Biolog and 16S rDNA sequence analysis showed that BRF-1 and BRF-2 were Paenibacillus polymyxa and Bacillus subtilis, respectively. |
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| Keywords: | Paenibacillus polymyxa Bacillus subtilis biolog identification 16S rDNA sequence biological characteristic |
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