| 柞蚕卵巢细胞原代培养及对核型多角体病毒的敏感性 |
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| 引用本文： | 刘淑珊,何龙. 柞蚕卵巢细胞原代培养及对核型多角体病毒的敏感性[J]. 蚕业科学, 1988, 0(4) |
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| 作者姓名： | 刘淑珊 何龙 |
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| 作者单位： | 辽宁省蚕业科学研究所(刘淑珊)，辽宁省蚕业科学研究所(何龙) |
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| 摘 要： | 对柞蚕卵巢细胞采用原代培养法,经6—7天形成细胞单层。对培养2周以上的细胞单层,进行柞蚕核型多角体病毒的感染试验,经2—3天可见部分细胞核内形成多角体,约半月感染率达60％左右。
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| 关 键 词： | 柞蚕 卵巢 细胞培养 核型多角体病毒 |
Primary Culture of Pupal Ovarian (Jells ot Antheraea pernyi and Its Suscep-tibility to ApNPV Infection, Sci |
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| Liu Shushan. Primary Culture of Pupal Ovarian (Jells ot Antheraea pernyi and Its Suscep-tibility to ApNPV Infection, Sci[J]. Acta Sericologica Sinica, 1988, 0(4) |
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| Authors: | Liu Shushan |
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| Affiliation: | Liu Shushan |
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| Abstract: | The pupal ovarian cells of Antheraea pernyi were cultured in Grace midium supplemented with 10% fetal bovine serum ( FBS ) and 3% hemoylmph of A.pernyi at pH 6.4. The cells migrated out and adhered to the bottom of the vessel and formed cell sheets after cultured 6-7 days.The morphology of the cells mainly showed round shape and the primary culture of ovarian cells could be maintained for more than 2 months. The celts were infected with nuclear poly-hedra could be seen in the nuclei of some cells after 4-7 days inoculation,The results indicated that the primary culture of the ovarian cells were susceptible to the nuclear polyhedrosis virus of A. pernyi. |
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| Keywords: | Ovary Anther aea pernyi Cell culture Nuclear polyhedra |
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