低温胁迫对甘薯S-腺苷甲硫氨酸合成酶mRNA表达水平的影响

S-腺苷甲硫氨酸合成酶（s-adenosylmethionine synthetase SAMS）是植物代谢中的一个关键酶，它催化甲硫氨酸与ATP生物合成S-腺苷甲硫氨酸（SAM）。SAM是生物合成乙烯以及多胺的前体，参与了植物的转甲基、转氨丙基、转硫反应等多种重要的生理过程。Frank等研究发现，SAM可以与RNA结合参与基因表达调控。近年来研究表明，乙烯和多胺都参与了植物抗逆反应，

Effect of Low Temperature Stress on Sweet Potato SAdenosyl Methionine Synthetase Gene Expression

Objective]The mRNA expression level changes of S-adenesylmethionine synthetase(SAMS)under low temperature stress was studied.Method]Total RNA were extracted from leaves,stem and earthnut of sweet potato 0,12,24,48 and 72 h after low temperature treatement,mRNA expression level was analyzed by reverse expression and Real-time PCR technique.Result]The expression quality of the gene extracted from leaves,stem and earthnut increased and the expression quality reached the peak point 24,72 and 72 h after low ~nperature treatment respectively.The expression change of earthnut was the biggest.Conclusion]Low temperature was good for increasing mRNA expression of relevart genes.