| 犬细小病毒北京分离株VP2基因的表达与抗原性分析 |
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| 引用本文： | 曾妮,李刚,朱鸿飞,侯绍华,史利军.犬细小病毒北京分离株VP2基因的表达与抗原性分析[J].中国畜牧兽医,2010,37(4):185-188. |
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| 作者姓名： | 曾妮 李刚 朱鸿飞 侯绍华 史利军 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所,北京,100193 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金(2009QN-3) |
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| 摘 要： | 本研究基于犬细小病毒(canine parvovirus,CPV)VP2基因序列设计引物,利用PCR技术扩增该基因。扩增的VP2基因连入表达载体pET-32a(+),经PCR及双酶切鉴定基因连入正确。重组表达载体转入大肠杆菌后,经IPTG诱导,SDS-PAGE及Western blotting分析表明有特异蛋白的表达,分子质量大小为87 ku。将表达的蛋白包被酶标板后用抗犬细小病毒单抗与其反应,表明表达的VP2蛋白可以与相应抗体特异结合。
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| 关 键 词： | 犬细小病毒 表达 抗原性 |
Prokaryotic Expression and Analysing Antigenicity of VP2 Gene Protein of CPV |
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| ZENG Ni,LI Gang,ZHU Hong-fei,HOU Shao-hua,SHI Li-jun.Prokaryotic Expression and Analysing Antigenicity of VP2 Gene Protein of CPV[J].China Animal Husbandry & Veterinary Medicine,2010,37(4):185-188. |
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| Authors: | ZENG Ni LI Gang ZHU Hong-fei HOU Shao-hua SHI Li-jun |
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| Institution: | Institute of Animal Science and Veterinary Medicine;Chinese Academy of Agricultural Sciences;Beijing 100193;China |
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| Abstract: | One specific pairs of primers,which were designed by canine parvovirus(CPV) VP2 gene sequence,were used to clone the VP2 gene isolated from Beijing through PCR.VP2 gene was subcloned from pEASY-T-VP2 into prokaryotic expression vector pET-32a.Sequence analysis confirmed that the VP2 gene was inserted correctly.SDS-PAGE and Western-blotting analysis revealed that the VP2 protein was expressed at high level in Escherichia coli.Through ELISA assay,the expressed VP2 protein has fine immunogenicity. |
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| Keywords: | canine parvovirus expression immunogenicity |
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