| 藻百年的组培快繁技术研究 |
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| 引用本文： | 张先云,袁秀云,马杰.藻百年的组培快繁技术研究[J].安徽农业科学,2009,37(11):4878-4879. |
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| 作者姓名： | 张先云 袁秀云 马杰 |
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| 作者单位： | 郑州师范高等专科学校生物技术研究所,河南郑州,450044;郑州师范高等专科学校生物技术研究所,河南郑州,450044;郑州师范高等专科学校生物技术研究所,河南郑州,450044 |
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| 基金项目： | 河南省郑州市重点科技攻关项目(051SGDS17017) |
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| 摘 要： | 目的]研究藻百年（Exacum tetragonum）的离体培养和快速繁殖技术。方法]利用藻百年的茎尖和茎段作为外植体进行组织培养和快速繁殖。结果]结果表明,MS＋6-BA2mg/L＋NAA0.2 mg/L＋蔗糖30 g/L＋琼脂8 g/L为最适诱导培养基;MS＋6-BA1 mg/L＋NAA0.1 mg/L＋蔗糖30 g/L＋琼脂8 g/L为最适增殖培养基,1/2 MS＋NAA0.3 mg/L＋蔗糖30 g/L＋琼脂8 g/L为最适生根培养基。结论]该研究可为其他龙胆科植物的组织培养与快速繁殖提供方法参考。
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| 关 键 词： | 藻百年 丛生芽 组织培养 快速繁殖 |
Study on Tissue Culture and Rapid Propagation of Exacum tetragonum |
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| ZHANG Xian-yun et al.Study on Tissue Culture and Rapid Propagation of Exacum tetragonum[J].Journal of Anhui Agricultural Sciences,2009,37(11):4878-4879. |
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| Authors: | ZHANG Xian-yun |
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| Institution: | ZHANG Xian-yun et al(Institute of Biology Technology,Zhengzhou Teacher\'s College,Zhengzhou,Henan 450044) |
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| Abstract: | Objective]The research aimed to study the tissue culture and rapid propagation of Exacum tetragonum.Method]The tissue culture and rapid propagation of E.tetragonum were carried out using stem apex and stem with buds as explants.Result]The results showed that MS+6-BA 2 mg/L+ NAA 0.2 mg/L + cane sugar 30 g/L+ agar 8 g/L was optimum medium for induction.MS+6-BA 1 mg/L+NAA 0.1 mg/L + cane sugar 30 g/L+ agar 8 g/L was the optimum medium for reduplication.1/2 MS+NAA 0.3 mg/L + cane sugar 30 g/L+ agar 8 g/L was... |
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| Keywords: | Exacum tetragonum Rosette bud Tissue culture Rapid propagation |
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