| SSR标记鉴定玉米品种亲子关系的研究 |
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| 引用本文： | 沈童伟,陆徐忠,刘勋辉,李莉,杨剑波. SSR标记鉴定玉米品种亲子关系的研究[J]. 农业科学与技术, 2009, 10(4): 55-60 |
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| 作者姓名： | 沈童伟 陆徐忠 刘勋辉 李莉 杨剑波 |
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| 作者单位： | 沈童伟,刘勋辉(安徽农业大学生命科学学院,安徽合肥,230036;安徽农业科学院水稻所,安徽合肥,230031);陆徐忠,李莉,杨剑波(安徽农业科学院水稻所,安徽合肥,230031) |
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| 基金项目： | the National Standard Plan (20051079-T-469).国家标准计划 |
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| 摘 要： | 1材料与方法1．1材料选取我国主要推广的16个玉米杂交组合及其双亲以及主要杂种优势群中的202个骨干自交系，其中185份自交系为中国玉米核心种质材料。种子严格隔离保纯，纯度99．9％以上。种子30℃发苗取其部分叶片提取DNA。
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| 关 键 词： | 玉米品种 亲子关系 标记鉴定 SSR 骨干自交系 材料选取 杂种优势群 杂交组合 |
Identification of the Parentage of Corn Variety Using SSR Markers |
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| SHEN Tong-wei,LU Xu-zhong,LIU Xun-hui,LI Li,YANG Jian-bo. Identification of the Parentage of Corn Variety Using SSR Markers[J]. Agricultural Science ＆ Technology, 2009, 10(4): 55-60 |
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| Authors: | SHEN Tong-wei LU Xu-zhong LIU Xun-hui LI Li YANG Jian-bo |
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| Affiliation: | 1. School of Life Sciences, Anhui Agricultural University, Hefei 230036; 2. Rice Research institute, Anhui Academy of Agricultural Sciences Hefei 230031) |
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| Abstract: | [Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primers, the artificial groups of two standard diad and two standard triad were respectively established as the verification materials. Genomic DNA of seedlings was extracted by using CTAB method. 137 pairs of SSR primers were selected for SSR amplification and product detection, which was used for the parentage identification of maize varieties. [ Result] Twenty pairs of corn primers with high polymorphism information content （ PIC value）, clearly amplified bands and good reproducibility were screened from 137 pairs of corn SSR primers tested. The identification results of using SSR molecular were consistent with the actual situation. [ Conclusion] It is feasible to identify the parentage of maize variety using SSR markers. |
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| Keywords: | Maize SSR marker Corn primer Parentage identification |
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