| 禽网状内皮组织增殖病病毒的实时荧光定量PCR检测 |
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| 引用本文： | 赵丽青,王树峰,吴延功,王志亮. 禽网状内皮组织增殖病病毒的实时荧光定量PCR检测[J]. 中国兽医学报, 2007, 27(6): 795-798 |
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| 作者姓名： | 赵丽青 王树峰 吴延功 王志亮 |
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| 作者单位： | 1. 山东出入境检验检疫局,山东,青岛,266002;农业部动物检疫所,山东,青岛,266032
2. 山东出入境检验检疫局,山东,青岛,266002 |
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| 摘 要： | 基于Light Cycler平台,利用SYBR GreenI染料能特异与双链DNA结合而发荧光的特性,建立了一种荧光PCR方法检测禽网状内皮组织增殖病病毒(REV)的方法。整个检测过程2 h内可以完成,病毒的最低检出浓度为10-7,在特异性试验中,REV有特异性的扩增曲线,而禽流感病毒和阴性对照一样,没有特异性扩增。对REV患鸡各器官组织进行了荧光PCR检测,各脏器病毒含量从高到低依次是肝、脾、腺胃、肾、肌胃,肝脏中的病毒含量最高,肌胃中的病毒含量最少,肺中没有检测到病毒。基于SYBR GreenI的荧光PCR方法检测REV快速、敏感、特异性好,为开展REV病原学检测提供了一种快速、无污染的方法。
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| 关 键 词： | 禽网状内皮组织增殖病病毒(REV) 检测 |
| 文章编号： | 1005-4545（2007）06-0795-04 |
| 修稿时间： | 2006-02-20 |
Research of dtection method of REV using real-time PCR |
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| ZHAO Li-qing,WANG Shu-feng,WU Yan-gong,WANG Zhi-liang. Research of dtection method of REV using real-time PCR[J]. Chinese Journal of Veterinary Science, 2007, 27(6): 795-798 |
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| Authors: | ZHAO Li-qing WANG Shu-feng WU Yan-gong WANG Zhi-liang |
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| Affiliation: | 1. Shangdong Entry-inspection and Quarantine Bureau, Qingdao, Shandong 266002, China; 2. National Exotic Animal Disease Center, Qingdao, Shandong 266032 |
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| Abstract: | Based on Light Cycler,using the conjunct speciality of SYBR GreenI dye and double-chain DNA,a detection method of REV using Real-time PCR.All detection course need 2h.The virus minimum detection concentration is 107.In the differential test,REV but AIV is detected.In the detection of sample,the viral content in liver is the highest,but no virus was detected in the lung.It would be a fast and no-pollution method for the pathogeny detection of REV. |
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| Keywords: | real-time PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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