半夏再生体系的建立

【目的】优化半夏再生条件,以获得优质半夏种苗.【方法】筛选出适宜的外植体后,使用3种激素2,4-D,KT,IAA利用正交设计法设置9个处理组对半夏愈伤组织进行诱导.选取长势良好,大小均一的半夏愈伤组织接于4种培养基中进行愈伤组织增殖.选取半夏叶柄诱导出的愈伤组织将其接于5种培养基中进行愈伤组织分化,观察不同培养基对半夏愈伤组织生根和出芽的影响,最后进行炼苗与移栽.【结果】在叶柄、叶片及块茎3种外植体中,叶柄是半夏愈伤组织诱导的最佳外植体;最有利于半夏愈伤组织诱导的培养基为MS+0.5 mg/L 2,4-D+1.5 mg/L KT+1.0 mg/L IAA;在暗培养条件下,将半夏愈伤组织继代于MS+2.0 mg/L 2,4-D+1.0 mg/L KT+1.0 mg/L IAA中生长最快;MS+0.2 mg/L 2,4-D+1.5 mg/L 6-BA培养基最有利于半夏愈伤组织的生根和出芽.

A Regeneration System of Pinellia ternate

Objective] This study was conducted to build a regeneration system of Pinellia ternate and optimize the regeneration conditions so as to obtain high-quality plantlets for cultivation.Methods] After screening out suitable explants of P. ternate, the experiment materials were divided into nine treatment groups according to the orthogonal design and treated with 2,4-D, KT and IAA to induce calli. Uniform healthy calli of P. ternate were selected and inoculated to four multiplication media. Petiole-derived calliwere selected and inoculated to five differentiation media. Then, the effects of different media on the rooting and sprouting of P. ternate calli were observed. Finally, the regenerated plantlets were hardened and transplanted.Results] Of the three explants studied (petiole, leaf and tuber), petiole was shown to be the best explant for callus induction. The best callus induction medium of P. ternate was MS+0.5 mg/L 2,4-D+1.5 mg/L KT+1.0 mg/L IAA. When culture was made in the dark, the best callus induction medium of P. ternate was MS+2.0 mg/L 2,4-D+1.0 mg/L KT+1.0 mg/L IAA. The best rooting medium of P. ternate was MS+0.2 mg/L 2,4-D+1.5 mg/L 6-BA.