Antiviral activity and underlying molecular mechanisms of Matrine against porcine reproductive and respiratory syndrome virus in vitro |
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| Authors: | Na Sun Zhi-Wei Wang Cai-Hong Wu E Li Jun-Ping He Shao-Yu Wang Yuan-Liang Hu Hai-Min Lei Hong-Quan Li |
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| Institution: | 1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China;2. Jiangsu Animal Husbandry & Veterinary College, Taizhou, Jiangsu 225300, PR China;3. University of Western Sydney, School of Medicine, Locked Bag 1797, Penrith 2751, NSW, Australia;4. Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China;5. School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, PR China |
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| Abstract: | Porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV), is an acute infectious disease. The prevalence of PRRS has made swine industry suffered huge financial losses. Matrine, a natural compound, has been demonstrated to possess anti-PRRSV activity in Marc-145 cells. However, the underlying molecular mechanisms were still unknown. The main objective of our study was to discuss the effect of Matrine on PRRSV N protein expression and PRRSV induced apoptosis. Indirect immunofluorescence assay (IFA) and Western blot were used to assess the effect of Matrine on N protein expression. Apoptosis was analyzed by fluorescence staining. In addition, the effect of Matrine on caspase-3 activation was investigated by Western blot. Indirect immunofluorescence assay and Western blot analysis demonstrated that Matrine could inhibit N protein expression in Marc-145 cells. And Matrine was found to be able to impair PRRSV-induced apoptosis by inhibiting caspase-3 activation. |
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| Keywords: | Matrine Antiviral PRRSV N protein Apoptosis |
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