Catalase addition to vitrification solutions maintains goat ovarian preantral follicles stability |
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| Authors: | AA Carvalho LR Faustino CMG Silva SV Castro CH Lobo FW Santos RR Santos CC Campello V Bordignon JR Figueiredo APR Rodrigues |
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| Institution: | 1. Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil;2. Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, CE, Brazil;3. Laboratory of Reproduction Biotechnology (BIOTECH), Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, Brazil;4. Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands;5. Animal Science Post-graduation Program, Federal University of Pará, Belém, PA, Brazil;6. Department of Animal Science, McGill University, Ste-Anne-de-Bellevue, QC, Canada |
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| Abstract: | The aim of this study was to verify whether the addition of catalase (20 IU/mL) at different steps of goat ovarian tissue vitrification affects ROS levels, follicular morphology and viability, stromal cell density, apoptosis and the expression of proteins related to DNA–damage signaling (γH2AX) and repair (53BP1). Goat ovarian tissues were analyzed fresh (control) or after vitrification: without catalase (VS–/WS–), with catalase in vitrification solutions (VS+/WS–), with catalase in washing solutions (VS–/WS+) or with catalase in both solutions (VS+/WS+). The vitrification without catalase had higher ROS levels than the control. The catalase, regardless the step of addition, maintained ROS levels similar to the control. There were no difference between treatments regarding follicular viability, stromal cell density and detection of γH2AX and 53BP1. There was no difference in follicular morphology and DNA fragmentation between groups vitrified. In conclusion, catalase addition to vitrification solutions prevents ROS formation in cryopreserved goat ovarian tissues. |
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| Keywords: | Cryopreservation Ovary ROS levels DNA damage Apoptosis |
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