Comparison of two real-time RT-PCR assays for differentiation of C-strain vaccinated from classical swine fever infected pigs and wild boars |
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| Authors: | F. Widé n,H. Everett,S. Blome,J. Fernandez Pinero,Å . Uttenthal,M. Cortey,T. von Rosen,M. Tignon,L. Liu |
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| Affiliation: | 1. Department of Virology, Immunobiology and Parasitology, The National Veterinary Institute (SVA), Uppsala, Sweden;2. Virology Department, Animal Health and Veterinary Laboratories Agency (AHVLA), Addlestone, UK;3. Institute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), Greifswald-Insel Riems, Germany;4. Centro de Investigación en Sanidad Animal (CISA-INIA), Madrid, Spain;5. National Veterinary Institute, Technical University of Denmark (DTU-VET), Lindholm, Kalvehave, Denmark;6. Veterinary and Agrochemical Research Centre CODA-CERVA, Uccle, Brussel, Belgium |
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| Abstract: | Classical swine fever is one of the most important infectious diseases for the pig industry worldwide due to its economic impact. Vaccination is an effective means to control disease, however within the EU its regular use is banned owing to the inability to differentiate infected and vaccinated animals, the so called DIVA principle. This inability complicates monitoring of disease and stops international trade thereby limiting use of the vaccine in many regions. The C-strain vaccine is safe to use and gives good protection. It is licensed for emergency vaccination in the EU in event of an outbreak. Two genetic assays that can distinguish between wild type virus and C-strain vaccines have recently been developed. Here the results from a comparison of these two real-time RT-PCR assays in an interlaboratory exercise are presented. Both assays showed similar performance. |
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| Keywords: | C-strain Vaccine PCR CSFV Classical swine fever DIVA |
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