云南省南苜蓿和天蓝苜蓿根瘤菌BOX-PCR分析

目的]进行南苜蓿和天蓝苜蓿根瘤菌的BOX-PCR分析，为苜蓿高效固氮根瘤菌的筛选和接种剂的研究提供理论依据。方法]采用boxAlR单引物对90株根瘤菌供试菌株及5株标准菌株进行BOX-PCR分析，并进行聚类分析。结果]经过扩增得到了较多的条带，90株供试菌株共有31种BOX图谱类型。聚类分析结果显示，所有菌株在40％相似性水平上聚为一群；而在69％的相似性水平上，供试菌株可细分为7个遗传群，群1的菌株全部分离自云南省江川县路居镇天蓝苜蓿的根瘤，群3内的菌株都分离自德宏州盈江县的南苜蓿根瘤，并与标准菌株Enisfermeliloti USDA 1002^T聚在一群，在群4、5、6中，除SWF67340的宿主为南苜蓿外，其他菌株的宿主都为天蓝苜蓿，群7中，除SWF66320、SWF67343、SWF65116和SWF673704株菌的宿主为天蓝苜蓿外，其余菌株的宿主都为南苜蓿。结论】供试菌株具有丰富的遗传多样性，BOX-PCR能够区分它们之间的差异；大多数菌株按宿主及采集地聚群，但也有交叉。

Study on the BOX-PCR Analysis of Rhizobium in the Bur Clover and Black Medic in Yunnan Province

Objective] The theoretical basis of the research on the screening and inoculants of high-efficient nitrogen-fixing alfalfa rhizobium was provided through the BOX-PCR analysis of bur clover and black medic.Method] 90 experimental rhizobium strains and five standard rhizobium strains were tested with boxA1R single primer by means of BOX-PCR analysis and cluster analysis.Results] More bands were produced after the strains were amplified and there were 31 kinds of BOX type mappings in 90 experimental rhizobi...