设施栽培中‘春捷’桃糖积累与相关酶活性的变化

 以设施栽培中‘春捷’桃为试材, 研究了其果实发育过程中糖积累与蔗糖代谢相关酶- 酸性转化酶(A I) 、中性转化酶(N I) 、蔗糖合成酶( SS) 及蔗糖磷酸合成酶( SPS) 活性的关系。结果表明:果实发育早期, 果实中的可溶性糖主要是还原糖; 中后期, 还原糖维持在相对较低的水平, 非还原糖大量积累。在整个发育过程中, 蔗糖持续积累, 尤其在成熟期急剧增加, 而果糖和葡萄糖含量在果实发育前期呈下降趋势, 中后期维持在一个相对较低的水平。A I、N I在果实发育前期活性最强, 之后迅速下降, 中后期维持在较低水平; SS在果实发育前期分解方向活性很强, 后期以合成方向为主, 并在果实成熟期保持较高水平; 在果实发育过程中SPS活性呈上升趋势, 但上升幅度较小。葡萄糖含量与A I、N I、SS-h活性显著正相关; 果糖含量与A I、SS-h显著正相关; 蔗糖含量与SS-s活性显著正相关, 与SPS不相关。

Sugar Accumulation and Changes of Sucrose-metabolizing Enzyme Activities in Peach under Protected Culture

Sugar accumulation and changes of sucrose-metabolizing enzyme activities were studied during the fruit development of'Chunjie'peach(Prunus persica).Results showed that most soluble sugars had been reduced at early stage of fruit development,the reduced sugar maintained in a low level during mid and late stage of fruit development while non-reduced sugars accumulated.Sucrose content increased from the beginning to the end of fruit development and the content of glucose and fructose tended to decrease at early stage of fruit development and remained in a low level thereafter.The highest activities of acid invertase(AI)and neutral invertase(NI)at early stage of fruit development decreased with fruit development and maintained in a low level during mid and late stage of fruit development.During the early fruit development,the activity of sucrose synthase(SS-h)kept the highest level,while that of SS-s increased continuously with fruit development and maintained in a low level at late stage of fruit development.The activity of SPS increased continuously,but the change of activity of SPS was less than that of SS.A significantly positive correlation was found between glucose content and activities of AI,NI and SS-h,and between fructose content and AI and SS-h activities.The content of sucrose was positively correlated with the activity of SS-s and no any correlation with the activity of SPS was found.