|
|||||
|
|
| Development and Evaluation of Codon-modified cry V Constructs in Cultivated Potato (Solanum tuberosum L. ) for Control of Potato Tuber Moth | |
| 作者姓名： | Guan Hongju Li Wenbin Douches David Lu Cuihua |
| 作者单位： | Department of |
| 基金项目： | Tacking key scientific and technical problems in Heilongjiang(GB04B101) |
| 摘 要： | A cryV gene,specifically toxic to Lepidoptera and Coleoptera,was incorporated into binary vectors with different promoters and the presence or absence of the β-glucuronidase(gus) reporter gene.These constructs were integrated into potato cv.Spunta by Agrobacterium tumefaciens-mediated transformation.Highest expression of cryV gene,determined by mRNA levels and insect mortality,was obtained using the CaMV 35S promoter without the gus gene configuration.Detached leaf and tuber bioassays showed a mortality rate of up to 83% and 100%,respectively,for potato tuber moth(Phthorimaea operculella Zeller) in the transgenic lines.Our results demonstrated that the presence of the gus gene negatively affects the expression level of the cryV gene.Bt expression was also facilitated by using the(ocs)3 mas super promoter,whereas the Bt expression regulated by the patatin promoter(tuber-specific) was too low to have any effect upon the mortality of potato tuber moth.These results represent significant improvement in the level of host plant resistance for the control of potato tuber moth via Bt transgenes. |
| 关 键 词： | 马铃薯块茎 马铃薯病毒 预防措施 基因 |
| 文章编号： | 1672-3635(2007)02-0065-09 |
| 修稿时间： | 2007年1月6日 |
Development and Evaluation of Codon-modified cry V Constructs in Cultivated Potato (Solanum tuberosum L. ) for Control of Potato Tuber Moth |
|
| Guan Hongju,Li Wenbin,Douches David,Lu Cuihua.Development and Evaluation of Codon-modified cry V Constructs in Cultivated Potato (Solanum tuberosum L. ) for Control of Potato Tuber Moth[J].Chinese Potato,2007,21(2):65-73. | |
| Authors: | Guan Hongju Li Wenbin Douches David Lu Cuihua |
| Institution: | 1. Huachuan Agrotechnique Extension Center, Huachuan, Heilongjiang 154300, China 2. College of Agronomy, Northeast Agricultural University, Harbin, Heilongjiang 150030, China 3. Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824, USA |
| Abstract: | A cryV gene, specifically toxic to Lepidoptera and Coleoptera, was incorporated into binary vectors with different promoters and the presence or absence of the β -glucuronidase (gus) reporter gene. These constructs were integrated into potato cv. Spunta by Agrobacterium tumefaciens-mediated transformation. Highest expression of cryV gene, determined by mRNA levels and insect mortality, was obtained using the CaMV 35S promoter without the gus gene configuration.Detached leaf and tuber bioassays showed a mortality rate of up to 83% and 100%, respectively, for potato tuber moth (Phthorimaea operculella Zeller) in the transgenic lines. Our results demonstrated that the presence of the gus gene negatively affects the expression level of the cryV gene. Bt expression was also facilitated by using the (ocs)3 mas super promoter, whereas the Bt expression regulated by the patatin promoter (tuber-specific) was too low to have any effect upon the mortality of potato tuber moth. These results represent significant improvement in the level of host plant resistance for the control of potato tuber moth via Bt transgenes. |
| Keywords: | potato tuber moth Bacillus thuringiensis gus gene promoter transgenic plant |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |