| 利用电泳谱带法鉴定蔬菜品种纯度的研究 |
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| 引用本文： | 王贵余. 利用电泳谱带法鉴定蔬菜品种纯度的研究[J]. 西北农业学报, 2005, 14(4): 100-102,112 |
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| 作者姓名： | 王贵余 |
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| 作者单位： | 聊城大学园艺系,山东,聊城,252000 |
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| 摘 要： | 以茄子、辣椒、南瓜、黄瓜、大白菜、萝卜等6种蔬菜为试材，研究了蛋白质聚丙烯酰胺凝胶电泳（PAGE）和EST、POD、AES等3种同工酶聚丙烯酰胺凝胶电泳技术在品种纯度鉴定上的应用效果。结果表明：辣椒、南瓜、黄瓜可采用蛋白质PAGE。大白菜、萝卜、辣椒采用同工酶PAGE进行品种纯度鉴定。同时，对电泳应用技术环节进行了研究和改进，确定了不同蔬菜作物的电泳技术。
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| 关 键 词： | 品种纯度 电泳 同工酶 蛋白质 蔬菜种子 |
| 文章编号： | 1004-1389（2005）04-0100-03 |
| 收稿时间： | 2005-03-14 |
| 修稿时间： | 2005-03-142005-04-28 |
Studies on Electrophoresis Method for the Cultivars Identification of Vegetable Seeds |
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| WANG Gui-yu. Studies on Electrophoresis Method for the Cultivars Identification of Vegetable Seeds[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2005, 14(4): 100-102,112 |
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| Authors: | WANG Gui-yu |
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| Affiliation: | Department of Horticuhure,Liaocheng University,Liaocheng Shandong 252000,China |
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| Abstract: | Researches have been carried out during 2000-2004 on eultivars purity identifieation of eggplant, pumpkin, eueumber, pepper, Chinese eabbage, radish by PAGE method of seed protein and isozymes. The result showed that all varieties of erops involved in this researeh ean be distinguished respeetively exeept eggplant. Protein PAGE method suited pumpkin, eueumber, pepper and isozymes PAGE method suited pepper, Chinese eabbage, radish. The eleetrophoresis method has also been improved in several links. |
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| Keywords: | Variety purity Eleetrophoresis Isozyme Protein Vegetable seeds |
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