| 百合鳞叶DNA提取及RAPD分析 |
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| 引用本文： | 童巧珍,周日宝,刘湘丹,盛孝邦,王朝晖.百合鳞叶DNA提取及RAPD分析[J].中南林业科技大学学报,2010,30(1). |
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| 作者姓名： | 童巧珍 周日宝 刘湘丹 盛孝邦 王朝晖 |
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| 作者单位： | 童巧珍(湖南农业大学,湖南,长沙,410128;湖南中医药大学,湖南,长沙,410208);周日宝,刘湘丹,王朝晖(湖南中医药大学,湖南,长沙,410208);盛孝邦(湖南农业大学,湖南,长沙,410128) |
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| 基金项目： | 国家科技支撑项目，湖南省中医药管理局项目 |
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| 摘 要： | 以百合新鲜鳞叶为材料,利用RAPD分子标记对来自我国5省共16份百合样品进行了分析。结果表明:采用改良CTAB法提取百合鳞叶中DNA条带清晰较,且从120条随机引物中筛选出35条有效引物,共得到769个扩增位点,其中628个位点具有多态性,占81.7%。聚类分析表明,16个百合材料在阈值为0.940 7时分为2个RAPD群,即分别属于百合科的百合属和大百合属;阈值为0.579 0时,百合属分为3个种,即分别为百合、卷丹和细叶百合。这些均可为进一步研究百合的分子生物学特性打下基础,为鉴定中药材百合品种品系的新方法提供充分的实验依据。
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| 关 键 词： | 分子生物学 百合 鳞叶 |
DNA extraction from scale leaf of Lily and analysis by RAPD |
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| TONG Qiao-zhen,ZHOU Ri-bao,LIU Xiang-dan,SHENG Xiao-bang,WANG Zhao-hui.DNA extraction from scale leaf of Lily and analysis by RAPD[J].Journal of Central South Forestry University,2010,30(1). |
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| Authors: | TONG Qiao-zhen ZHOU Ri-bao LIU Xiang-dan SHENG Xiao-bang WANG Zhao-hui |
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| Institution: | TONG Qiao-zhen1,2,ZHOU Ri-bao2,LIU Xiang-dan2,SHENG Xiao-bang1,WANG Zhao-hui2(1.Hunan Agricultural University,Changsha 410128,Hunan,China,2.Hunan University of Chinese Medicine,Changsha 410208,China) |
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| Abstract: | In this study,16 samples of Lily collected from different ecological sources were analyzed by RAPD using its leaf scales.The bands of DNA extracted by ameliorated CTAB.Thirty-five primers were screened from 120 random primers,and a total 769 DNA bands were amplified,628(81.7%) of which were polymorphic.Cluster analysis results show that the 16 Lily materials were divided into two RAPD groups at 0.940 7.There are two different genera plants: Lilium and Cardiocrinum;Lilium was divided into three different typ... |
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| Keywords: | DNA RAPD molecular biology Lily scale leaf DNA RAPD |
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