| 牛分枝杆菌RecA基因的克隆与序列分析 |
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| 引用本文： | 王春芳,郭伟生,姜秀云,钱爱东.牛分枝杆菌RecA基因的克隆与序列分析[J].湖北农业科学,2009,48(10). |
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| 作者姓名： | 王春芳 郭伟生 姜秀云 钱爱东 |
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| 作者单位： | 1. 吉林农业大学,动物科技学院,长春,130118
2. 吉林农业大学,生命科学学院,长春,130118 |
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| 基金项目： | 国家科技支撑计划项目 |
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| 摘 要： | 以牛分枝杆菌(Mycobacterium bovis)Valleelll株染色体DNA为模板,以RecA基因特异性引物进行PCR扩增,获得约240 bp的DNA片段.将PCR产物克隆至pMDTM18-T Vector中,通过α-互补法筛选、质粒PCR鉴定及序列分析鉴定,成功构建出了重组质粒pMDTM18-T-RecA,为进一步研究RecA基因及其在牛结核病诊断中的应用奠定了基础.
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| 关 键 词： | 牛分枝杆菌 RecA基因 克隆 序列分析 |
Cloning and Sequential Analysis of Mycobacterium bovis RecA Gene |
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| WANG Chun-fang,GUO Wei-sheng,JIANG Xiu-yun,QIAN Ai-dong.Cloning and Sequential Analysis of Mycobacterium bovis RecA Gene[J].Hubei Agricultural Sciences,2009,48(10). |
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| Authors: | WANG Chun-fang GUO Wei-sheng JIANG Xiu-yun QIAN Ai-dong |
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| Abstract: | The chromosome DNA was extracted from Mycobacterium bovis Valleelll,the RecA gene was amplified with a pair of specific primers by using polymerase chain reaction (PCR). The PCR product was approximately 240 bp DNA segment. The PCR product was cloned into pMD?8-T Vector,then by using a-complementation test,PCR assay and recom-binant plasmid sequential analysis,recombinant plasmid pMD?8-T-RecA was successfully constructed. These results could serve as a solid basis for further studies on the usefulness of RecA gene and its application in diagnosis of tuberculosis. |
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| Keywords: | Mycobacterium bovis RecA gene cloning sequential analysis |
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