Improving controlled pollination methodology for breeding <Emphasis Type="Italic">Acacia mangium</Emphasis> Willd. |
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Authors: | A R Griffin Tran Duc Vuong J L Harbard C Y Wong C Brooker R E Vaillancourt |
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Institution: | (1) School of Plant Science, University of Tasmania, Private Bag 55, Hobart, TAS, 7001, Australia;(2) Riau Andelan Pulp & Paper, Kerinci, Indonesia;(3) Forest Science Institute of Vietnam, Hanoi, Vietnam |
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Abstract: | Acacia mangium is a major plantation species for the pulp and paper industry in south-east Asia and there are a number of active breeding
programs. The species is predominantly outcrossing, but with a demonstrated capacity to set selfed seed where outcross pollen
is limited, with consequent inbreeding depression in the progeny. Current controlled pollination methods therefore include
a time-consuming emasculation step. We used microsatellite genotyping of seedlings to determine the consequences of outcross
pollination with and without emasculation. Only 1 of 3 mother trees set a small amount (5%) of selfed seed. Using whole inflorescences
from the male parent as the pollen applicator rather than sieved pollen reduced outcross contamination rates from 19.1 to
8.7% and substantially increased worker productivity. Application of sugar solution to the female flowers immediately prior
to pollination increased yield of sound seeds per spike. Additional improvements to the pollination protocols are discussed. |
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