| 应用单克隆抗体ＡＣ—ＥＬＩＳＡ检测鸡蛋卵黄中鸡毒支原体 |
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| 引用本文： | 杨百亮,赵翠萍,刘田生,李天俊.应用单克隆抗体ＡＣ—ＥＬＩＳＡ检测鸡蛋卵黄中鸡毒支原体[J].中国预防兽医学报,2001,23(1):50-52. |
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| 作者姓名： | 杨百亮 赵翠萍 刘田生 李天俊 |
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| 作者单位： | 天津农学院动科系, |
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| 基金项目： | 天津市教委科技计划项目(96307B) |
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| 摘 要： | 采用抗鸡毒支原体阳性血清包被酶标板作为捉抗体，以４株鸡毒支原体特异单克隆抗体作为第二抗体和酶标羊抗鼠ＩgG作为指示体建立了一种检测鸡蛋卵黄中鸡毒支原体的双夹心ＡＣ－ＥＬＩＳＡ。该法具有简便、灵敏、快速等优点，从采样到获得结果６小时内完成，检出率高于分离培养法，且重复性良好，该方法的建立鸡群毒支原体感染的检测和净化提供了一种新的可靠方法。
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| 关 键 词： | 鸡毒支原体 单克隆抗体 鸡蛋黄 AC－ELISA 检测 |
| 文章编号： | 1008-0589(2001)01-0050-03 |
| 修稿时间： | 2000年6月5日 |
Establishment of A
Monoclonal Antibody-based AC-ELISA to Detect M.gallisepticum in Egg Yolk |
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| YANG Bailiang,Zhao Cuiping,LIU Tianshen,LI Tianjun.Establishment of A
Monoclonal Antibody-based AC-ELISA to Detect M.gallisepticum in Egg Yolk[J].Chinese Journal of Preventive Veterinary Medicine,2001,23(1):50-52. |
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| Authors: | YANG Bailiang Zhao Cuiping LIU Tianshen LI Tianjun |
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| Abstract: | An antigen-capture ELISA(AC-ELISA) was developed for titration of M.gallisepticum in egg yolk.The plates were coated with polyclonal anti-M.gallisepticum serum and reacted with M.gallisepticum antigens,which were detected with species-specific monoclonal antibodies to M.gallisepticum,followed by anti-rat enzyme-labelled conjugate antiserum(IgG).The procedure in detecting the bacteria in egg yolk was accomplished in 6 hours,and showed to be more sensitive than the conventional culture method.Egg yolk samples collected from eggs were tested for M.gallisepticum by the method and showed a positive rate of 62%.The results demonstrated that the AC-ELISA has rapid,sensitive and simple advantages for monitoring chicken flocks and detecting M.gallisepticum in eggs. |
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| Keywords: | M gallisepticum Monoclonal antibody Egg yolk AC_ELISA Detection |
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