| 苏云金芽胞杆菌毒素蛋白和粘虫颗粒体病毒对甜菜夜蛾中肠围食膜的破坏作用 |
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| 引用本文： | 刘琴,马谈斌,祁建杭,施建德,李传明,徐健.苏云金芽胞杆菌毒素蛋白和粘虫颗粒体病毒对甜菜夜蛾中肠围食膜的破坏作用[J].中国生物防治,2011,27(2):182-187. |
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| 作者姓名： | 刘琴 马谈斌 祁建杭 施建德 李传明 徐健 |
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| 作者单位： | 江苏里下河地区农业科学研究所,扬州,225007 |
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| 基金项目： | 国家自然科学基金(31071740); 江苏省自然科学基金(BK2009702,BK2010295); 江苏省333高层次人才培养项目 |
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| 摘 要： | 利用扫描电镜和SDS-PAGE技术研究了苏云金芽胞杆菌(Bacillus thuringiensis,Bt)、粘虫颗粒体病毒(PuGV-Ps)及其增效蛋白(enhancin)对甜菜夜蛾Spodoptera exigua中肠围食膜的影响.电镜观察结果表明,正常的甜菜夜蛾围食膜外壁有韧性,表面较平滑,少皱褶,内壁表面较粗糙,有较厚质感,无孔洞和缝隙;取食PuGV-Ps或增效蛋白后的围食膜外壁皱缩,内壁质薄;Bt单独作用于围食膜同样改变了围食膜结构,但影响程度较小;未发现不同处理对甜菜夜蛾围食膜造成穿孔或裂缝.SDS-PAGE结果表明,取食PuGV-Ps或增效蛋白后,围食膜上150kD蛋白一定程度被降解,27kD蛋白也被部分降解,而29kD蛋白被完全降解;取食Bt后的围食膜上29kD蛋白也被降解.离体降解试验进一步证明PuGV-Ps增效蛋白对甜菜夜蛾围食膜上多种蛋白具有降解作用,甜菜夜蛾围食膜上29kD蛋白是Bt和PuGV-Ps增效蛋白共同作用的靶标蛋白.
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| 关 键 词： | 粘虫颗粒体病毒 苏云金杆菌 围食膜 甜菜夜蛾 |
Damaging Effects of PuGV-Ps and Bacillus thuringiensis on Peritrophic Membrane of Spodoptera exigua |
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| LIU Qin
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MA Tan-bin
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QI Jian-hang
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SHI Jian-de
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LI Chuan-ming
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XU Jian.Damaging Effects of PuGV-Ps and Bacillus thuringiensis on Peritrophic Membrane of Spodoptera exigua[J].Chinese Journal of Biological Control,2011,27(2):182-187. |
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| Authors: | LIU Qin MA Tan-bin QI Jian-hang SHI Jian-de LI Chuan-ming XU Jian |
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| Institution: | LIU Qin,MA Tan-bin,QI Jian-hang,SHI Jian-de,LI Chuan-ming,XU Jian*(Jiangsu Lixiahe Institute of Agricultural Science,Yangzhou 225007,China) |
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| Abstract: | Effect of Bacillus thuringiensis(Bt),Pseudaletia unipuncta granulovirus-Ps(PuGV-Ps) and PuGV-Ps enhancin on peritrophic membrane(PM) of Spodoptera exigua was studied by scanning electron microscope and SDS-PAGE.Scanning electron microscope observation indicated that the exterior surface of S.exigua PM was smooth with few rumples and the interior surface of PM was thick texture with some granulation.There were no holes and cracks in the membrane.When PuGV-Ps or enhancin was fed,the exterior surface of PM shr... |
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| Keywords: | Pseudaletia unipuncta granulovirus-Ps Bacillus thuringiensis peritrophic membrane Spodoptera exigua |
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