| ILTV gB基因与鸡IL-18基因共表达DNA疫苗的免疫原性 |
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| 引用本文： | 刘金朋,崔沛,陈红英,邵攀峰,王淑娟,朱前磊,王子馨. ILTV gB基因与鸡IL-18基因共表达DNA疫苗的免疫原性[J]. 中国兽医学报, 2011, 31(4) |
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| 作者姓名： | 刘金朋 崔沛 陈红英 邵攀峰 王淑娟 朱前磊 王子馨 |
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| 作者单位： | 1. 河南农业大学,牧医工程学院,河南郑州450002
2. 河南省动物疫病预防控制中心,河南郑州,450008 |
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| 基金项目： | 国家“十一五”科技支撑计划专项(2006BAD06A08) |
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| 摘 要： | 本试验构建了能分别或同时表达ILTV gB和IL-18基因的重组质粒pIRES-gB、pIRES-IL18、pIRES-gB/IL18。将重组质粒通过腿部肌肉多点注射免疫21日龄雏鸡,35日龄加强免疫1次,二免后2周用ILTV HN1株进行攻毒。pIRES-gB/IL18和pIRES-gB+pIRES-IL18免疫雏鸡后均能促进外周血T淋巴细胞亚群数量和血清中特异性抗体水平的增加,能明显增强ILTV DNA疫苗对强毒的攻击保护,但pIRES-gB/IL18免疫组要优于pIRES-gB+pIRES-IL18混合免疫组及其他对照组,差异显著或极显著。结果表明,鸡IL-18能明显增强ILTV DNA疫苗的免疫原性。
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| 关 键 词： | 传染性喉气管炎病毒 gB基因 鸡IL-18 DNA疫苗 |
Enhancement of DNA vaccine potency against ILTV by coexpression of ILTV gB and IL-18 gene |
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| LIU Jin-peng,CUI Pei,CHEN Hong-ying,SHAO Pan-feng,WANG Shu-juan,ZHU Qian-lei,WANG Zi-xin. Enhancement of DNA vaccine potency against ILTV by coexpression of ILTV gB and IL-18 gene[J]. Chinese Journal of Veterinary Science, 2011, 31(4) |
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| Authors: | LIU Jin-peng CUI Pei CHEN Hong-ying SHAO Pan-feng WANG Shu-juan ZHU Qian-lei WANG Zi-xin |
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| Affiliation: | LIU Jin-peng1,CUI Pei2,CHEN Hong-ying1*,SHAO Pan-feng1,WANG Shu-juan1,ZHU Qian-lei1,WANG Zi-xin1(1.College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou 450002,China,2.Henan Province Center for Animal Diseases Control and Prevention,Zhengzhou 450008,China) |
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| Abstract: | The gB gene of avian infectious laryngotracheitis virus(ILTV) and chicken interleukin-18(IL-18) gene from chicken were inserted into the bicistronic pIRES plasmid.The pIRES-gB,pIRES-IL18 and pIRES-gB/IL18 plasmid expressing or co-expressing gB and IL-18 gene were constructed.The 21-day-old chickens were immunized intramuscularly with plasmids and boosted two weeks later.Two weeks after boosting,chickens were challenged by virulent ILTV strain.The results showed that co-administration of a plasmid expressing... |
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| Keywords: | infectious laryngotracheitis virus gB gene chicken IL-18 DNA vaccine |
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