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| 猪圆环病毒2型Cap蛋白特异性抗原表位重组腺病毒的构建及鉴定 | |
| 引用本文： | 郭全海,陈陆,王川庆,吴德峰,王永生,李志娟,王艳.猪圆环病毒2型Cap蛋白特异性抗原表位重组腺病毒的构建及鉴定[J].中国兽医学报,2011,31(8):1099-1102,1110. |
| 作者姓名： | 郭全海 陈陆 王川庆 吴德峰 王永生 李志娟 王艳 |
| 作者单位： | 1. 河南农业大学牧医工程学院,河南郑州450002／商丘职业技术学院,河南商丘476000／福建农林大学,福建福州350002 2. 河南农业大学牧医工程学院,河南郑州,450002 3. 福建农林大学,福建福州,350002 |
| 基金项目： | 国家高技术研究与发展计划项目(2007AA100606); 河南省高等学校青年骨干教师计划资助项目(2010GGJS-045) |
| 摘 要： | 猪圆环病毒2型PCV2ORF2基因表达的Cap蛋白含有病毒的主要抗原表位,是PCV2基因工程苗研究的主要候选蛋白。根据GenBank公布的PCV2基因序列设计1对PCR引物,从感染有PCV2的PK-15细胞中扩增出包含2个特异性表位的Cap蛋白基因片段。把Cap蛋白基因目的基因克隆入腺病毒5型穿梭质粒中,构成重组穿梭质... |
| 关 键 词： | 猪圆环病毒2型(PCV2) Cap蛋白基因(ORF2基因) 腺病毒 鉴定 |
Construction and Indentification of Recombinant Adenoviruses Containing the ORF2 Gene of Porcine Circovirus 2 |
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| GUO Quan-hai,,CHEN Lu,WANG Chuan-qing,WU De-feng,WANG Yong-sheng,LI Zhi-juan,WANG Yan.Construction and Indentification of Recombinant Adenoviruses Containing the ORF2 Gene of Porcine Circovirus 2[J].Chinese Journal of Veterinary Science,2011,31(8):1099-1102,1110. | |
| Authors: | GUO Quan-hai CHEN Lu WANG Chuan-qing WU De-feng WANG Yong-sheng LI Zhi-juan WANG Yan |
| Institution: | GUO Quan-hai1,2,3,CHEN Lu1,WANG Chuan-qing1,WU De-feng3,WANG Yong-sheng1,LI Zhi-juan1,WANG Yan1(1.College of Animal Science and Veterinary Medicine,Henan Agriculture University,Zhengzhou 450002,China,2 Shangqiu Vocational and Technical College,Shangqiu,Henan 476000,3 Fujian Agriculture and Forestry University,Fuzhou 350002,China) |
| Abstract: | Expressing protein contains govering antigeon epitopes of porcine circovirus serotype 2 gene,which is reaschered widely for genetic engineering vaccine.Aaccording to Genbank,designed a pair of primers which can amplify Cap protein gene contained two specific epitopes from PK-15 affected PCV2 by PCR.The gene was subcloned into the shuttle vector pShuttle-CMV of adenoviruses serotype 5 constructed recombinant shuttle plasmid rpShuttle-CMV-276.Backbone vector pAdeasy-1 and rpShuttle-CMV-276 transformed into co... |
| Keywords: | Porcine circovirus serotype 2 Cap protein gene(ORF2 gene) Recombinant adenoviruses Identification |
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