| 绵羊Klf4基因逆转录病毒载体的构建与检测 |
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| 引用本文： | 王春生,杨越飞,宁方勇,薛超,朴善花,安铁洙. 绵羊Klf4基因逆转录病毒载体的构建与检测[J]. 中国兽医学报, 2011, 31(8): 1118-1122 |
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| 作者姓名： | 王春生 杨越飞 宁方勇 薛超 朴善花 安铁洙 |
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| 作者单位： | 东北林业大学生命科学学院,黑龙江哈尔滨,150040 |
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| 基金项目： | 国家自然科学基金资助项目(30771538,31000990); 中央高校基本科研业务费专项资金资助项目(DL10BA06) |
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| 摘 要： | Klf4作为诱导产生多能性干细胞的基因之一,在体细胞的重编程过程中发挥着重要的作用。为构建绵羊Klf4基因逆转录病毒载体,参照本实验室已克隆出的绵羊Klf4基因编码区（GenBank登录号GQ280389）设计引物,将扩增的基因片段经过酶切后亚克隆到逆转录病毒载体pMXs的多克隆位点,经过酶切、PCR和测序鉴定正确后,...
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| 关 键 词： | 绵羊 Klf4基因 逆转录病毒载体 |
Construction and detection of retrovirus vector with sheep Klf4 gene |
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| WANG Chun-sheng,YANG Yue-fei,NING Fang-yong,XUE Chao,PIAO Shan-hua,AN Tie-zhu. Construction and detection of retrovirus vector with sheep Klf4 gene[J]. Chinese Journal of Veterinary Science, 2011, 31(8): 1118-1122 |
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| Authors: | WANG Chun-sheng YANG Yue-fei NING Fang-yong XUE Chao PIAO Shan-hua AN Tie-zhu |
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| Affiliation: | WANG Chun-sheng,YANG Yue-fei,NING Fang-yong,XUE Chao,PIAO Shan-hua,AN Tie-zhu(College of Life Science,Northeast Forestry University,Harbin 150040,China) |
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| Abstract: | Klf4 as one of the genes for inducing pluripotent stem cells,plays an important role in the somatic cell reprogramming.To construct the retrovirus vector with sheep Klf4,this experiment refered to the coding region of sheep Klf4 gene sequence,which had cloned by our laboratory(GenBank accession No:GQ280389).Then we artificially synthesized primers by the restriction enzyme digesting and connected with pMXs retrovirus vector.After identifying,recombinant plasmid was correctly detected by PCR and restriction ... |
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| Keywords: | sheep Krüppel-like gene 4 retrovirus vector |
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