| 酵母双杂交诱饵载体pGBKT7_MYC2构建及表达鉴定 |
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| 引用本文： | 刘武,肖牧,阮颖,刘春林. 酵母双杂交诱饵载体pGBKT7_MYC2构建及表达鉴定[J]. 作物研究, 2012, 26(2): 143-147 |
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| 作者姓名： | 刘武 肖牧 阮颖 刘春林 |
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| 作者单位： | 1. 湖南农业大学生物科学技术学院,长沙410128;作物种质创新和资源利用国家重点实验室培育基地,长沙410128
2. 湖南农业大学农学院,长沙410128;作物种质创新和资源利用国家重点实验室培育基地,长沙410128 |
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| 基金项目： | 国家自然科学基金面上项目(31071455) |
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| 摘 要： | MYC2是一类含有helix - loop - helix (bHLH)结构域的转录因子.为进一步研究MYC2因子在植物防御抗性中的作用及其参与植物JA,SA等信号途径的作用机制,克隆了拟南芥的MYC2基因,以此构建了pGBKT7_MYC2酵母双杂交载体,通过Western blotting验证表明,该载体能在酵母细胞里正常表达.
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| 关 键 词： | MYC2基因 pGBKT7_MYC2 诱饵载体 蛋白表达 |
Construction and Expressional Identification of Yeast Two- hybrid Bait Expression Vector pGBKT7_ MYC2 |
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| LIU Wu , XIAO Mu , RUAN Ying , LIU Chun-lin. Construction and Expressional Identification of Yeast Two- hybrid Bait Expression Vector pGBKT7_ MYC2[J]. Crop Research, 2012, 26(2): 143-147 |
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| Authors: | LIU Wu XIAO Mu RUAN Ying LIU Chun-lin |
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| Affiliation: | 1 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China; 2 College of Agronomy, Hunan Agricultural University, Changsha, Hunan 410128, China; 3 Pre - state Key Laboratory of Crop Germplasm Innovation and Utilization, Changsha, Hunan 410128, China) |
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| Abstract: | MYC2, a basic helix- loop -helix （bHLH） domain - containing TF, acts as a positive regulator of abscisic acid - dependent drought responses and is also induced in JA - mediated responses. Takirrg the cDNA from Arabidophsis as the template, full - length CDS of MYC2 had been cloned and then was ligated into the bait expression vector pG- BKT7. After verified by digestion, the bait vector was transformed into Glod yeast cells, and the expression of MYC2 gene was checked by Western blotting. As a result, the bait expression vector pGBKT7_ MYC2 was constructed successfully, which laid the foundation for screening target proteins interacting and mapping the network with the MYC2 protein. |
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| Keywords: | MYC2 gene pGBKTT_MYC2 Bait vector Protein expression |
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