紫花苜蓿下胚轴再生条件的优化

[目的]优化紫花苜蓿下胚轴的再生条件。[方法]选用不同的培养基和激素，对公农1号、公农2号、美国苜蓿王、草原1号4个苜蓿品种的下胚轴进行愈伤组织的诱导和分化。[结果]经筛选，获得了诱导愈伤组织的基本培养基为改良SH＋MS有机＋MS微量及铁盐＋2，4-D2．0mg/L＋KT0．05mg／L＋蔗糖30g／L，诱导再生植株分化的培养基为MS＋NAA1．0mg／L＋KT0．1mg／L＋水解酪蛋白25mg／L＋蔗糖20g／L。[结论]得到公农1号、公农2号、草原1号3个易于诱导和分化的基因型。

Optimization of the Regeneration Conditions from Alfalfa Hypocotyls

[Objective] The research aimed to optimize the regeneration conditions of hypocotyls alfalfa.[Method] Different media and hormones were used to induce and differentiate from the hypocotyls on Gongnong 1, Gongnong 2, American Alfaking and Caoyuan 1.[Result] After screening, the basic medium for inducing the callus was obtained as: improved SH+MS organic fractions +MS micronutrients + 2.0 mg/L 2,4-D + 0.05 mg/L KT+ 30 g/L sucrose.The medium for inducing the differentiation of regenerated plants was obtained as MS+1.0 mg/L NAA +0.1 mg/L KT+25 mg/L casein hydrolysate and 20 g/L sucrose.[Conclusion] Three genotypes for the induction and differentiation were obtained from Gongnong 1, Gongnong 2, American Alfaking and Caoyuan 1.