| 水稻圆粒基因RS的鉴定和定位 |
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| 引用本文： | 陈雅萍,缪荣,刘喜,陈本佳,兰杰,马腾飞,王益华,刘世家,江玲. 水稻圆粒基因RS的鉴定和定位[J]. 作物学报, 2019, 45(1): 1-9. DOI: 10.3724/SP.J.1006.2019.82032 |
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| 作者姓名： | 陈雅萍 缪荣 刘喜 陈本佳 兰杰 马腾飞 王益华 刘世家 江玲 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心 |
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| 基金项目： | This study was supported by the National Key Research and Development Program of China(2016YFD0100101-08);the Key Projects of National Natural Science Foundation(91535302);the National University Student Innovation Program(201710307014) |
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| 摘 要： | 阐明水稻籽粒大小相关基因的遗传和分子机制对水稻产量形成具有重要意义。利用甲基磺酸乙酯(ethyl methanesulfonate, EMS)诱变粳稻品种宁粳3号筛选获得圆粒突变体round seed (rs)。遗传分析表明,突变体rs圆粒表型由单隐性核基因控制。颖壳扫描电镜观察发现,rs籽粒变圆主要是细胞数目改变导致的。在突变体rs中,细胞周期相关基因的表达较野生型显著升高。将RS定位在第3染色体短臂标记RM3413与N3-5之间,物理距离约589 kb。RS突变影响BR信号途径,改变了粒型相关基因的表达。本研究有助于阐明水稻籽粒发育的分子机制。
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| 收稿时间： | 2018-06-07 |
Identification and mapping of round seed related gene in rice (Oryza sativa L.) |
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| Ya-Ping CHEN,Rong MIAO,Xi LIU,Ben-Jia CHEN,Jie LAN,Teng-Fei MA,Yi-Hua WANG,Shi-Jia LIU,Ling JIANG. Identification and mapping of round seed related gene in rice (Oryza sativa L.)[J]. Acta Agronomica Sinica, 2019, 45(1): 1-9. DOI: 10.3724/SP.J.1006.2019.82032 |
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| Authors: | Ya-Ping CHEN Rong MIAO Xi LIU Ben-Jia CHEN Jie LAN Teng-Fei MA Yi-Hua WANG Shi-Jia LIU Ling JIANG |
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| Affiliation: | State Key Laboratory of Crop Genetics and Germplasm Enhancement / Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China |
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| Abstract: | It is significant to clarify the genetic and molecular mechanism of genes related to rice grain size for rice yield. A mutant round seed (rs) was screened from japonica variety “Ningjing 3” mutagenized by ethyl methane sulfonate (EMS). Genetic analysis revealed that the phenotype of rs was controlled by a single recessive nuclear gene. Scanning electron microscope observation indicated that the change of cell number was responsible for the mutant phenotype of rs. Compared with the wild type, the expression of cell cycle related-genes increased significantly in rs. RS was located in the 589 kb region of chromosome 3, between markers RM3413 and N3-5. The RS mutation affected BR signal pathway, and changed the expression of grain size-related genes. This research contributes to elucidating the molecular mechanism of rice grain development. |
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| Keywords: | rice (Oryza sativa L.) round seed mutant phenotypic analysis gene mapping |
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