小麦新种质CH1357抗白粉病遗传分析及染色体定位

白粉病是影响小麦产量和品质的一种主要病害。小偃麦衍生品系CH1357对白粉病具有较好的成株抗性,苗期对27个菌株表现为免疫或高抗,是一个高抗白粉病的优异抗源。为了明确其抗白粉病基因在染色体上的位置,对台长29/CH1357和绵阳11/CH1357的F_1、BC_1及F_(2:3)家系进行了遗传分析,并利用分离群体分组分析法(bulked segregantanalysis,BSA)将其初步定位。CH1357的白粉病抗性受1对显性核基因控制,位于染色体5DS,暂命名为PmCH1357。其侧翼连锁标记为Xcfd81和Xbwm8,在2个作图群体台长29/CH1357和绵阳11/CH1357中的遗传距离分别为2.0 cM/11.3 cM和1.5 cM/8.9 cM。PmCH1357与5DS染色体上已报道的其他抗白粉病基因抗谱不同,可能是一个新的抗源。

Genetic analysis and chromosomal localization of powdery mildew resistance gene in wheat germplasm CH1357

Powdery mildew is a serious disease affecting yield and quality of wheat. Wheat-Thinopyrum imtermedium introgression line CH1357 is highly resistant to Blumeria graminis f. sp. tritici (Bgt) at the adult plant stage and immune or highly resistant to 27 Bgt isolates at the seedling stage. Two mapping populations (F₁, BC₁, and F_2:3) derived from Taichang 29/CH1357 and Mianyang 11/CH1357 were used to map the powdery mildew resistance gene by the bulked segregant analysis. The powdery mildew resistance in CH1357 was controlled by a single dominant gene, temporarily named PmCH1357. This gene was located on the short arm of chromosome 5D and linked to SSR markers Xcfd81 and Xbwm8, with genetic distances of 2.0 cM and 11.3 cM in the Taichang 29/CH1357 population and 1.5 cM and 8.9 cM in the Mianyang 11/CH1357 population, respectively. PmCH1357 differs from other Pm genes reported on chromosome 5DS in resistance spectrum, which may be a new source of resistance.