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玉米丝黑穗病苗期快速鉴定方法
引用本文:王振华,刘长华,张林,李新海. 玉米丝黑穗病苗期快速鉴定方法[J]. 植物保护, 2009, 35(6): 99-103. DOI: 10.3969/j.issn.0529-1542.2009.06.022
作者姓名:王振华  刘长华  张林  李新海
作者单位:1. 东北农业大学,哈尔滨,150030
2. 中国农业科学院作物科学研究所,北京,100081
基金项目:国际科技合作项目,黑龙江省青年学术骨干教师支持计划项目 
摘    要:本试验以已知抗性的51份玉米自交系为材料,研究了苗期接菌条件下可溶性糖含量与田间发病率的关系。结果表明,在15/25 ℃变温及1%土壤接菌条件下,幼苗可溶性糖含量与田间发病率存在极显著的正相关(r=0.799 4)。采用逻辑斯蒂模型分别建立了抗、感自交系可溶性糖含量与田间发病率的一元曲线回归方程:y=12.084 8/[1+exp (6.372 5-0.367 5x)]和y=91.017 0/[1+exp(18.448 8-0.788 8x)],以此建立了以接菌幼苗可溶性糖含量为指标的抗性评价方法,并用于未知抗性种质资源的鉴定。

关 键 词:玉米  丝黑穗病  苗期  鉴定方法

Rapid evaluation method for head smut at seedling stage of maize
Wang Zhenhua,Liu Changhua,Zhang Lin,Li Xinhai. Rapid evaluation method for head smut at seedling stage of maize[J]. Plant Protection, 2009, 35(6): 99-103. DOI: 10.3969/j.issn.0529-1542.2009.06.022
Authors:Wang Zhenhua  Liu Changhua  Zhang Lin  Li Xinhai
Affiliation:1. Northeast Agricultural University, Harbin150030, China;2. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing100081, China
Abstract:The relationships between soluble sugar content and disease percentage were analyzed in 51 inbred lines at seedling stage with artificial inoculation.The significant positive correlation(r=0.799 4)was found between the soluble sugar content and disease percentage under the conditions of 15/25℃ and 1% soil bacteria.Using logistic model,regression equations were established as followed:y=12.084 8/[1+exp(6.372 5-0.367 5x)]and y=91.017 0/[1+exp(18.448 8-0.788 8x)],which was used to evaluate the responses of maize inbred lines to head smut resistance.
Keywords:maize  head smut  seedling stage  evaluation method
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