| 百合病毒脱毒及检测技术研究进展 |
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| 引用本文： | 王丽花,王继华,瞿素萍,杨秀梅,吴学尉. 百合病毒脱毒及检测技术研究进展[J]. 广西农业科学, 2007, 38(6): 673-677 |
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| 作者姓名： | 王丽花 王继华 瞿素萍 杨秀梅 吴学尉 |
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| 作者单位： | 1. 云南省农业科学院农业部花卉产品质量监督检验测试中心,昆明,650205
2. 云南省农业科学院花卉研究所,昆明,650205 |
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| 基金项目： | 云南省科技攻关项目(2004NG08) |
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| 摘 要： | 阐述百合病毒的种类和危害,系统地介绍热处理、茎尖培养、珠芽培养、热处理结合茎尖培养、病毒抑制剂结合茎尖培养等5种脱毒方法,以及利用指示植物、电镜技术、酶联免疫和分子生物学技术检测百合主要病毒的方法。
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| 关 键 词： | 百合 病毒 脱毒 检测 |
| 文章编号： | 1002-8161（2007）06-0673-05 |
| 收稿时间： | 2007-09-30 |
| 修稿时间： | 2007-09-30 |
Advances in research of eradication and detection of viruses in lily |
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| WANG Li-hua,WANG Ji-hua,QU Su-ping,YANG Xiu-mei,WU Xue-wei. Advances in research of eradication and detection of viruses in lily[J]. Guangxi Agricultural Sciences, 2007, 38(6): 673-677 |
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| Authors: | WANG Li-hua WANG Ji-hua QU Su-ping YANG Xiu-mei WU Xue-wei |
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| Abstract: | The species of lily viruses and its damage were expatiated.Five virus-free methods including thermotherapy,meristem culture in vitro,bulblet culture,thermotherapy combined with meristem culture in vitro,chemotherapy combined with meristem culture in vitro,and the main virus-detecting methods such as indicator plants,electron microscope,enzyme linked immunosobent assay(ELISA),techniques of molecular biology were systematically introduced. |
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| Keywords: | lily virus virus-free detection |
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